Cigarette smoke inhalation impairs angiogenesis in early bone healing processes and delays fracture union

C. J. Chang, I. M. Jou, T. T. Wu, F. C. Su, T. W. Tai

研究成果: Article同行評審

15 引文 斯高帕斯(Scopus)


Aims Cigarette smoking has a negative impact on the skeletal system, causes a decrease in bone mass in both young and old patients, and is considered a risk factor for the development of osteoporosis. In addition, it disturbs the bone healing process and prolongs the healing time after fractures. The mechanisms by which cigarette smoking impairs fracture healing are not fully understood. There are few studies reporting the effects of cigarette smoking on new blood vessel formation during the early stage of fracture healing. We tested the hypothesis that cigarette smoke inhalation may suppress angiogenesis and delay fracture healing. Methods: We established a custom-made chamber with airflow for rats to inhale cigarette smoke continuously, and tested our hypothesis using a femoral osteotomy model, radiograph and microcT imaging, and various biomechanical and biological tests. Results: In the smoking group, Western blot analysis and immunohistochemical staining revealed less expression of vascular endothelial growth factor (VeGF) and von Willebrand factor (vWF). The smoking group also had a lower microvessel density than the control group. Image and biochemical analysis also demonstrated delayed bone healing. Conclusion: Cigarette smoke inhalation was associated with decreased expression of angiogenic markers in the early bone healing phase and with impaired bone healing.

頁(從 - 到)99-107
期刊Bone and Joint Research
出版狀態Published - 2020

All Science Journal Classification (ASJC) codes

  • 手術
  • 骨科和運動醫學


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