Codon usage regulates protein structure and function by affecting translation elongation speed in Drosophila cells

Fangzhou Zhao, Chien Hung Yu, Yi Liu

研究成果: Article同行評審

50 引文 斯高帕斯(Scopus)

摘要

Codon usage biases are found in all eukaryotic and prokaryotic genomes and have been proposed to regulate different aspects of translation process. Codon optimality has been shown to regulate translation elongation speed in fungal systems, but its effect on translation elongation speed in animal systems is not clear. In this study, we used a Drosophila cell-free translation system to directly compare the velocity of mRNA translation elongation. Our results demonstrate that optimal synonymous codons speed up translation elongation while non-optimal codons slow down translation. In addition, codon usage regulates ribosome movement and stalling on mRNA during translation. Finally, we show that codon usage affects protein structure and function in vitro and in Drosophila cells. Together, these results suggest that the effect of codon usage on translation elongation speed is a conserved mechanism from fungi to animals that can affect protein folding in eukaryotic organisms.

原文English
頁(從 - 到)8484-8492
頁數9
期刊Nucleic acids research
45
發行號14
DOIs
出版狀態Published - 2017 8月 21

All Science Journal Classification (ASJC) codes

  • 遺傳學

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