Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells

Jin Yuan Shih, Shuenn Chen Yang, Tse Ming Hong, Ang Yuan, Jeremy J.W. Chen, Chong Jen Yu, Yih Leong Chang, Yung Chie Lee, Konan Peck, Cheng Wen Wu, Pan Chyr Yang

研究成果: Article

130 引文 (Scopus)

摘要

Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P = .010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P = .016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.

原文English
頁(從 - 到)1392-1400
頁數9
期刊Journal of the National Cancer Institute
93
發行號18
DOIs
出版狀態Published - 2001 九月 19

指紋

Neoplasm Metastasis
Neoplasms
Lung Neoplasms
Genes
Northern Blotting
collapsin response mediator protein-1
Reverse Transcription
Western Blotting
Telophase
Suppressor Genes
Polymerase Chain Reaction
Centrosome
Spindle Apparatus
Pseudopodia
Interphase
Fluorescence Microscopy
Mitosis
Cytoplasm
Lymph Nodes
Observation

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

引用此文

Shih, Jin Yuan ; Yang, Shuenn Chen ; Hong, Tse Ming ; Yuan, Ang ; Chen, Jeremy J.W. ; Yu, Chong Jen ; Chang, Yih Leong ; Lee, Yung Chie ; Peck, Konan ; Wu, Cheng Wen ; Yang, Pan Chyr. / Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells. 於: Journal of the National Cancer Institute. 2001 ; 卷 93, 編號 18. 頁 1392-1400.
@article{e839d38c2d09471aaf19c11860c42ae7,
title = "Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells",
abstract = "Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P = .010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P = .016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.",
author = "Shih, {Jin Yuan} and Yang, {Shuenn Chen} and Hong, {Tse Ming} and Ang Yuan and Chen, {Jeremy J.W.} and Yu, {Chong Jen} and Chang, {Yih Leong} and Lee, {Yung Chie} and Konan Peck and Wu, {Cheng Wen} and Yang, {Pan Chyr}",
year = "2001",
month = "9",
day = "19",
doi = "10.1093/jnci/93.18.1392",
language = "English",
volume = "93",
pages = "1392--1400",
journal = "Journal of the National Cancer Institute",
issn = "0027-8874",
publisher = "Oxford University Press",
number = "18",

}

Shih, JY, Yang, SC, Hong, TM, Yuan, A, Chen, JJW, Yu, CJ, Chang, YL, Lee, YC, Peck, K, Wu, CW & Yang, PC 2001, 'Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells', Journal of the National Cancer Institute, 卷 93, 編號 18, 頁 1392-1400. https://doi.org/10.1093/jnci/93.18.1392

Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells. / Shih, Jin Yuan; Yang, Shuenn Chen; Hong, Tse Ming; Yuan, Ang; Chen, Jeremy J.W.; Yu, Chong Jen; Chang, Yih Leong; Lee, Yung Chie; Peck, Konan; Wu, Cheng Wen; Yang, Pan Chyr.

於: Journal of the National Cancer Institute, 卷 93, 編號 18, 19.09.2001, p. 1392-1400.

研究成果: Article

TY - JOUR

T1 - Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells

AU - Shih, Jin Yuan

AU - Yang, Shuenn Chen

AU - Hong, Tse Ming

AU - Yuan, Ang

AU - Chen, Jeremy J.W.

AU - Yu, Chong Jen

AU - Chang, Yih Leong

AU - Lee, Yung Chie

AU - Peck, Konan

AU - Wu, Cheng Wen

AU - Yang, Pan Chyr

PY - 2001/9/19

Y1 - 2001/9/19

N2 - Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P = .010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P = .016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.

AB - Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P = .010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P = .016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.

UR - http://www.scopus.com/inward/record.url?scp=0035913647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035913647&partnerID=8YFLogxK

U2 - 10.1093/jnci/93.18.1392

DO - 10.1093/jnci/93.18.1392

M3 - Article

C2 - 11562390

AN - SCOPUS:0035913647

VL - 93

SP - 1392

EP - 1400

JO - Journal of the National Cancer Institute

JF - Journal of the National Cancer Institute

SN - 0027-8874

IS - 18

ER -