Comparative nutritional and chemical phenome of Clostridium difficile isolates determined using phenotype microarrays

Joy Scaria, Jenn Wei Chen, Nicodemus Useh, Hongxuan He, Sean P. McDonough, Chunhong Mao, Bruno Sobral, Yung Fu Chang

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)


Objectives: Clostridium difficile infection (CDI) is the leading cause of infectious diarrhea in North America and Europe. The risk of CDI increases significantly in the case where antimicrobial treatment reduces the number of competing bacteria in the gut, thus leading to the increased availability of nutrients and loss of colonization resistance. The objective of this study was to determine comprehensive nutritional utilization and the chemical sensitivity profile of historic and newer C. difficile isolates and to examine the possible role of the phenotype diversity in C. difficile virulence. Methods: Phenotype microarrays (PMs) were used to elucidate the complete nutritional and chemical sensitivity profile of six C. difficile isolates. Results: Of the 760 nutrient sources tested, 285 compounds were utilized by at least one strain. Among the C. difficile isolates compared, R20291, a recent hypervirulent outbreak-associated strain, appears to have an expanded nutrient utilization profile when compared to all other strains. Conclusions: The expanded nutritional utilization profile of some newer C. difficile strains could be one of the reasons for infections in patients who are not exposed to the hospital environment or not undergoing antibiotic treatment. This nutritional profile could be used to design tube feeding formulas that reduce the risk of CDI.

頁(從 - 到)20-25
期刊International Journal of Infectious Diseases
出版狀態Published - 2014 10月 1

All Science Journal Classification (ASJC) codes

  • 微生物學(醫學)
  • 傳染性疾病


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