Complete nucleotide sequence of the intergenic spacer between 25S and 17s rDNA in Miscanthus sinensis var. glaber

The nucleotide sequence of the intergenic spacer (IGS) between the 25S and 17S ribosomal RNA genes of Miscanthus sinensis var. glaber was determined. The spacer length (2,178 bp) in Miscanthus is close to that of rice IGS (2,140 bp). Many repeated elements with consensus lengths of 34 and 59 base pairs are found in the nontranscribed region (NTS), upstream of the putative transcription initiation site (TIS). Subrepeats B with four copies are longer and better conserved than subrepeats A with ten copies. The sequences of the external transcribed region (ETS) show high identity, ranging from 66% to 76%, among Miscanthus, rice, maize, and wheat.