Composition-dependent protein secretion and integrin level of osteoblastic cell on calcium silicate cements

Ming You Shie, Hsien Chang Chang, Shinn Jyh Ding

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28 引文 斯高帕斯(Scopus)

摘要

The purpose of this study was to investigate the responses of the human osteosarcoma cell line MG63 to calcium silicate cements with different Si/Ca molar ratios and different surface roughness. In particular, the study evaluated integrin subunit levels, phosphor-focal adhesion kinase (pFAK) levels and protein production at the cell attachment stage. The results indicated that the surface roughness (variations within a factor of 10) of the cements did not play a prominent role in cell attachment and proliferation, but the effect of composition was highlighted. Increased pFAK and total integrin levels and promoted cell attachment and cell cycle progression were observed upon an increase in cement Si content. Cement with a higher Si content was beneficial for collagen Type I (COL I) adsorption, COL I secretion, and αlibβ3 subintegrin expression, whereas cement with a higher Ca content increased fibronectin (FN) adsorption, FN secretion, and enhanced αvβ1 subintegrin levels. These results establish composition-dependent differences in integrin binding as a mechanism regulating cellular responses to biomaterial surfaces. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 769-780, 2014.

原文English
頁(從 - 到)769-780
頁數12
期刊Journal of Biomedical Materials Research - Part A
102
發行號3
DOIs
出版狀態Published - 2014 三月 1

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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