Conjunctival expression of toll-like receptor 3 plays a pathogenic role in the formation of ultraviolet light-induced pterygium

Chun Chieh Lai, Sung Huei Tseng, Sheng Min Hsu, Yin Ting Huang, Chi Chang Shieh

研究成果: Article同行評審

2 引文 斯高帕斯(Scopus)

摘要

PURPOSE. Toll-like receptor 3 (TLR3), as a damage-associated molecular pattern sensor, can detect self-RNA released from necrotic cells induced by ultraviolet B (UVB) radiation exposure. Pterygium formation is believed to be a tumorigenesis-like process induced by UVB exposure. In this study, we aimed to investigate the expression pattern of TLR3 in pterygium specimens and cultured pterygial epithelial cells (PECs). METHODS. Human pterygium and ipsilateral pterygium-free conjunctiva from the same patients were used in this study. The expression of TLR3 and nuclear factor-kappa B (NF-κB) was investigated in these specimens. PECs were exposed to UVB radiation to determine the effect of UVB on the expression of TLR3 and the activation of NF-κB. RESULTS. The immunofluorescence study showed stronger TLR3 expression in superficial epithelial cells in the pterygial epithelium in comparison with the normal conjunctival epithelium. The expression of TLR3 decreased in intensity from the superficial epithelium toward the basal cell layer, implying a correlation between UVB exposure and TLR3 expression. Differential TLR3 expression patterns in pterygial and conjunctival tissues were also found in quantitative PCR analyses. PECs after UVB irradiation had higher protein levels of TLR3 and phospho-NF-κB than those of the PECs without irradiation. Immunofluorescence studies showed that UVB irradiation induced the nuclear translocation of NF-κB in the PECs. In PECs with the targeted TLR3 gene silencing, the expression of phospho-NF-κB was not induced by UVB irradiation. CONCLUSIONS. Our results indicate that UVB exposure, TLR3 expression, and NF-κB activation may be a critical sequence that leads to the formation of pterygium.

原文English
文章編號6
期刊Investigative Ophthalmology and Visual Science
62
發行號10
DOIs
出版狀態Published - 2021 8月

All Science Journal Classification (ASJC) codes

  • 眼科
  • 感覺系統
  • 細胞與分子神經科學

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