Constitutive overexpressed type I interferon induced downregulation of antiviral activity in medaka fish (Oryzias latipes)

Shun Maekawa, Takashi Aoki, Han Ching Wang

研究成果: Article同行評審

9 引文 斯高帕斯(Scopus)


In fish, as well as vertebrates, type I interferons (IFNs) are important cytokines that help to provide innate, antiviral immunity. Although low amounts of IFN are constitutively secreted under normal physiological conditions, long-term and excessive IFN stimulation leads to reduced sensitivity to the IFN signal. This provides a negative feedback mechanism that prevents inappropriate responses and autoimmunity. At present, however, neither IFN desensitization nor the normal physiological role of constitutive IFN are well characterized in fish. The objective here was therefore to produce and characterize a transgenic medaka fish (Oryzias latipes), designated IFNd-Tg, that constitutively overexpressed the IFNd gene. A dual promoter expression vector was constructed for overexpression of IFNd under an EF1α promoter and a DsRed reporter gene under control of a γF-crystaline promoter. The phenotype of the IFNd-Tg fish had a lower response to poly(I:C) and increased susceptibility to nervous necrosis virus (NNV) infection compared to wild-type (WT). Furthermore, transduction of IFN signals for STAT1b, STAT2 and IRF9 were down-regulated in the IFNd-Tg fish, and expression levels of RLR signal molecules (MDA5, MITA, IRF1 and IRF3) were lower than in WT. The constitutive overexpression of IFNd resulted in desensitization of IFN-stimulation, apparently due to downregulation of IFN signal transduction, and this caused increased susceptibility to NNV.

頁(從 - 到)12-20
期刊Developmental and Comparative Immunology
出版狀態Published - 2017 3月 1

All Science Journal Classification (ASJC) codes

  • 免疫學
  • 發展生物學


深入研究「Constitutive overexpressed type I interferon induced downregulation of antiviral activity in medaka fish (Oryzias latipes)」主題。共同形成了獨特的指紋。