Construction of consecutive deletions of the Escherichia coli chromosome

Jun Ichi Kato, Masayuki Hashimoto

研究成果: Article同行評審

129 引文 斯高帕斯(Scopus)

摘要

The minimal set of genetic information necessary and sufficient to sustain a functioning cell contains not only trans-acting genes, but also cis-acting chromosomal regions that cannot be complemented by plasmids carrying these regions. In Escherichia coli (E. coli), only one chromosomal region, the origin of replication has been identified to be cis-acting. We constructed a series of mutants with long-range deletions, and the chromosomal regions containing trans-acting essential genes were deleted in the presence of plasmids complementing the deleted genes. The deleted regions cover all regions of the chromosome except for the origin and terminus of replication. The terminus affects cell growth, but is not essential. Our results indicate that the origin of DNA replication is the only vital, unique cis-acting DNA sequence in the E. coli chromosome necessary for survival.

原文English
文章編號132
期刊Molecular Systems Biology
3
DOIs
出版狀態Published - 2007

All Science Journal Classification (ASJC) codes

  • 一般生物化學,遺傳學和分子生物學
  • 一般免疫學和微生物學
  • 一般農業與生物科學
  • 應用數學

指紋

深入研究「Construction of consecutive deletions of the Escherichia coli chromosome」主題。共同形成了獨特的指紋。

引用此