Copper response of Proteus hauseri based on proteomic and genetic expression and cell morphology analyses

I. Son Ng, Xuesong Zheng, Nan Wang, Bor Yann Chen, Xia Zhang, Yinghua Lu

研究成果: Article

5 引文 (Scopus)

摘要

The copper response of Proteus hauseri ZMd44 was determined using one-dimensional (1D) gel electrophoresis coupled with MALDI-TOF-TOF mass spectrometry for a similarity analysis of proteins isolated from P. hauseri ZMd44 cultured in CuSO4-bearing LB medium. Candidate proteins identified as a copper-transporting P-type ATPase (CTPP), phosphoenolpyruvate carboxykinase (PEPCK), flagellin (Fla), and outer membrane proteins (Omps) were the major copper-associated proteins in P. hauseri. In a comparative analysis of subcellular (i.e., periplasmic, intracellular, and inner membranes) and cellular debris, proteomics analysis revealed a distinct differential expression of proteins in P. hauseri with and without copper ion exposure. These findings were consistent with the transcription level dynamics determined using quantitative real-time PCR. Based on a genetic cluster analysis of copper-associated proteins from P. hauseri, Fla and one of the Omps showed greater diversity in their protein sequences compared to those of other Proteus species. Transmission electron microscopy (TEM) and the observed growth on LB agar plates showed that the swarming motility of cells was significantly suppressed and inhibited upon Cu(II) exposure. Thus, copper stress could have important therapeutic significance due to the loss of swarming motility capacity in P. hauseri, which causes urinary tract infections.

原文English
頁(從 - 到)1057-1072
頁數16
期刊Applied Biochemistry and Biotechnology
173
發行號5
DOIs
出版狀態Published - 2014 七月

指紋

Proteus
Proteomics
Copper
Proteins
Flagellin
Membrane Proteins
Bearings (structural)
Membranes
Intracellular Membranes
Phosphoenolpyruvate
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cluster analysis
Transcription
Electrophoresis
Transmission Electron Microscopy
Debris
Urinary Tract Infections
Agar
Cell Movement
Mass spectrometry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

引用此文

Ng, I. Son ; Zheng, Xuesong ; Wang, Nan ; Chen, Bor Yann ; Zhang, Xia ; Lu, Yinghua. / Copper response of Proteus hauseri based on proteomic and genetic expression and cell morphology analyses. 於: Applied Biochemistry and Biotechnology. 2014 ; 卷 173, 編號 5. 頁 1057-1072.
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Copper response of Proteus hauseri based on proteomic and genetic expression and cell morphology analyses. / Ng, I. Son; Zheng, Xuesong; Wang, Nan; Chen, Bor Yann; Zhang, Xia; Lu, Yinghua.

於: Applied Biochemistry and Biotechnology, 卷 173, 編號 5, 07.2014, p. 1057-1072.

研究成果: Article

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