Cordyceps sinensis mycelium induces MA-10 mouse leydig tumor cell apoptosis by activating the caspase-8 pathway and suppressing the NF-κB pathway

H. Y. Yang, S. F. Leu, Yang-Gao Wang, C. S. Wu, Bu-Miin Huang

研究成果: Article

19 引文 (Scopus)

摘要

□ Cordyceps sinensis has been used as nutritious food and medicine in Chinese society. CS can inhibit tumor growth and induce tumor cell apoptosis. CS induced MA-10 mouse Leydig tumor cell death, but the anti-tumor mechanisms are not fully understood. Thus, the aim of this study was to investigate the apoptotic effect of CS on MA-10 cells and determine the molecular mechanism. CS (2-10 mg/ml) was added to MA-10 cells at different time scales (0-24 h). The condensation of DNA chromatin and apoptotic nuclear fragmentation increased in CS-treated MA-10 cells. Western blot analysis showed that 3 hours of CS treatment caused an increase in caspase-3 and -8 expressions only, which provided further evidence for the involvement of caspase-3 and -8 in CS-induced MA-10-cell apoptosis. CS blocked NF-κ protein expression in a dose-dependent relationship. CS induces MA-10 cell apoptosis by activating caspase-8-dependent and caspase-9-independent pathways and downregulating NF-κB protein expression.

原文English
頁(從 - 到)103-110
頁數8
期刊Archives of Andrology
52
發行號2
DOIs
出版狀態Published - 2006 三月 1

指紋

Cordyceps
Leydig Cell Tumor
Caspase 8
Mycelium
Apoptosis
Caspase 3
Neoplasms
Caspase 9
Chromatin
Cell Death
Down-Regulation
Western Blotting
Medicine
Food
DNA
Growth

All Science Journal Classification (ASJC) codes

  • Endocrinology

引用此文

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title = "Cordyceps sinensis mycelium induces MA-10 mouse leydig tumor cell apoptosis by activating the caspase-8 pathway and suppressing the NF-κB pathway",
abstract = "□ Cordyceps sinensis has been used as nutritious food and medicine in Chinese society. CS can inhibit tumor growth and induce tumor cell apoptosis. CS induced MA-10 mouse Leydig tumor cell death, but the anti-tumor mechanisms are not fully understood. Thus, the aim of this study was to investigate the apoptotic effect of CS on MA-10 cells and determine the molecular mechanism. CS (2-10 mg/ml) was added to MA-10 cells at different time scales (0-24 h). The condensation of DNA chromatin and apoptotic nuclear fragmentation increased in CS-treated MA-10 cells. Western blot analysis showed that 3 hours of CS treatment caused an increase in caspase-3 and -8 expressions only, which provided further evidence for the involvement of caspase-3 and -8 in CS-induced MA-10-cell apoptosis. CS blocked NF-κ protein expression in a dose-dependent relationship. CS induces MA-10 cell apoptosis by activating caspase-8-dependent and caspase-9-independent pathways and downregulating NF-κB protein expression.",
author = "Yang, {H. Y.} and Leu, {S. F.} and Yang-Gao Wang and Wu, {C. S.} and Bu-Miin Huang",
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T1 - Cordyceps sinensis mycelium induces MA-10 mouse leydig tumor cell apoptosis by activating the caspase-8 pathway and suppressing the NF-κB pathway

AU - Yang, H. Y.

AU - Leu, S. F.

AU - Wang, Yang-Gao

AU - Wu, C. S.

AU - Huang, Bu-Miin

PY - 2006/3/1

Y1 - 2006/3/1

N2 - □ Cordyceps sinensis has been used as nutritious food and medicine in Chinese society. CS can inhibit tumor growth and induce tumor cell apoptosis. CS induced MA-10 mouse Leydig tumor cell death, but the anti-tumor mechanisms are not fully understood. Thus, the aim of this study was to investigate the apoptotic effect of CS on MA-10 cells and determine the molecular mechanism. CS (2-10 mg/ml) was added to MA-10 cells at different time scales (0-24 h). The condensation of DNA chromatin and apoptotic nuclear fragmentation increased in CS-treated MA-10 cells. Western blot analysis showed that 3 hours of CS treatment caused an increase in caspase-3 and -8 expressions only, which provided further evidence for the involvement of caspase-3 and -8 in CS-induced MA-10-cell apoptosis. CS blocked NF-κ protein expression in a dose-dependent relationship. CS induces MA-10 cell apoptosis by activating caspase-8-dependent and caspase-9-independent pathways and downregulating NF-κB protein expression.

AB - □ Cordyceps sinensis has been used as nutritious food and medicine in Chinese society. CS can inhibit tumor growth and induce tumor cell apoptosis. CS induced MA-10 mouse Leydig tumor cell death, but the anti-tumor mechanisms are not fully understood. Thus, the aim of this study was to investigate the apoptotic effect of CS on MA-10 cells and determine the molecular mechanism. CS (2-10 mg/ml) was added to MA-10 cells at different time scales (0-24 h). The condensation of DNA chromatin and apoptotic nuclear fragmentation increased in CS-treated MA-10 cells. Western blot analysis showed that 3 hours of CS treatment caused an increase in caspase-3 and -8 expressions only, which provided further evidence for the involvement of caspase-3 and -8 in CS-induced MA-10-cell apoptosis. CS blocked NF-κ protein expression in a dose-dependent relationship. CS induces MA-10 cell apoptosis by activating caspase-8-dependent and caspase-9-independent pathways and downregulating NF-κB protein expression.

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