Crowning proteins: Modulating the protein surface properties using crown ethers

Cheng Chung Lee, Manuel Maestre-Reyna, Kai Cheng Hsu, Hao Ching Wang, Chia I. Liu, Wen Yih Jeng, Li Ling Lin, Richard Wood, Chia Cheng Chou, Jinn Moon Yang, Andrew H.J. Wang

研究成果: Article同行評審

49 引文 斯高帕斯(Scopus)


Crown ethers are small, cyclic polyethers that have found wide-spread use in phase-transfer catalysis and, to a certain degree, in protein chemistry. Crown ethers readily bind metallic and organic cations, including positively charged amino acid side chains. We elucidated the crystal structures of several protein-crown ether co-crystals grown in the presence of 18-crown-6. We then employed biophysical methods and molecular dynamics simulations to compare these complexes with the corresponding apoproteins and with similar complexes with ring-shaped low-molecular-weight polyethylene glycols. Our studies show that crown ethers can modify protein surface behavior dramatically by stabilizing either intra- or intermolecular interactions. Consequently, we propose that crown ethers can be used to modulate a wide variety of protein surface behaviors, such as oligomerization, domain-domain interactions, stabilization in organic solvents, and crystallization.

頁(從 - 到)13054-13058
期刊Angewandte Chemie - International Edition
出版狀態Published - 2014 11月 24

All Science Journal Classification (ASJC) codes

  • 催化
  • 一般化學


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