Crystal Structures of a Piscine Betanodavirus: Mechanisms of Capsid Assembly and Viral Infection

Nai Chi Chen, Masato Yoshimura, Hong Hsiang Guan, Ting Yu Wang, Yuko Misumi, Chien Chih Lin, Phimonphan Chuankhayan, Atsushi Nakagawa, Sunney I. Chan, Tomitake Tsukihara, Tzong Yueh Chen, Chun Jung Chen

研究成果: Article

49 引文 斯高帕斯(Scopus)

摘要

Betanodaviruses cause massive mortality in marine fish species with viral nervous necrosis. The structure of a T = 3 Grouper nervous necrosis virus-like particle (GNNV-LP) is determined by the ab initio method with non-crystallographic symmetry averaging at 3.6 Å resolution. Each capsid protein (CP) shows three major domains: (i) the N-terminal arm, an inter-subunit extension at the inner surface; (ii) the shell domain (S-domain), a jelly-roll structure; and (iii) the protrusion domain (P-domain) formed by three-fold trimeric protrusions. In addition, we have determined structures of the T = 1 subviral particles (SVPs) of (i) the delta-P-domain mutant (residues 35−217) at 3.1 Å resolution; and (ii) the N-ARM deletion mutant (residues 35−338) at 7 Å resolution; and (iii) the structure of the individual P-domain (residues 214−338) at 1.2 Å resolution. The P-domain reveals a novel DxD motif asymmetrically coordinating two Ca2+ ions, and seems to play a prominent role in the calcium-mediated trimerization of the GNNV CPs during the initial capsid assembly process. The flexible N-ARM (N-terminal arginine-rich motif) appears to serve as a molecular switch for T = 1 or T = 3 assembly. Finally, we find that polyethylene glycol, which is incorporated into the P-domain during the crystallization process, enhances GNNV infection. The present structural studies together with the biological assays enhance our understanding of the role of the P-domain of GNNV in the capsid assembly and viral infection by this betanodavirus.

原文English
文章編號e1005203
期刊PLoS pathogens
11
發行號10
DOIs
出版狀態Published - 2015 一月 1

    指紋

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

引用此

Chen, N. C., Yoshimura, M., Guan, H. H., Wang, T. Y., Misumi, Y., Lin, C. C., Chuankhayan, P., Nakagawa, A., Chan, S. I., Tsukihara, T., Chen, T. Y., & Chen, C. J. (2015). Crystal Structures of a Piscine Betanodavirus: Mechanisms of Capsid Assembly and Viral Infection. PLoS pathogens, 11(10), [e1005203]. https://doi.org/10.1371/journal.ppat.1005203