Crystallographic structure of Ni-Co coating on the affinity adsorption of histidine-tagged protein

Yaw Jen Chang, Sheng Zheng Chen, Ching Yuan Ho

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)

摘要

The principle of immobilized metal affinity chromatography (IMAC) has been recently implemented for protein microarrays for the study of protein abundance and function. Ni-Co film fabricated by electrodeposition is a novel microarray surface in an alloy type for immobilizing histidine-tagged proteins based on IMAC. In this paper, the effects of crystallographic structures and surface properties of Ni-Co coatings, with and without the annealing process, on the immobilization of histidine-tagged proteins were systematically investigated. The experimental results reveal that the stronger hcp texture, due to a higher Co content, results in better affinity adsorption for histidine-tagged biotin. Nevertheless, the allotropic phase transformation from hcp to fcc, due to the annealing process, leads to the decrease of affinity adsorption. The wettability property and the surface roughness of Ni-Co coating are, however, not important factors. Obviously, the crystallographic structure of Ni-Co coating is the dominant factor for the specific affinity adsorption of histidine-tagged protein.

原文English
頁(從 - 到)55-60
頁數6
期刊Colloids and Surfaces B: Biointerfaces
128
DOIs
出版狀態Published - 2015 四月 1

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 表面和介面
  • 物理與理論化學
  • 膠體和表面化學

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