Cyclic stretch facilitates myogenesis in C2C12 myoblasts and rescues Thiazolidinedione-Inhibited Myotube Formation

Ya Ju Chang, Yun Ju Chen, Chia Wei Huang, Shih Chen Fan, Bu Miin Huang, Wen Tsan Chang, Yau Sheng Tsai, Fong Chin Su, Chia Ching Wu

研究成果: Article

9 引文 (Scopus)

摘要

Thiazolidinedione (TZD), a specific peroxisome proliferator-activated receptor γ (PPARγ) agonist, was developed to control blood glucose in diabetes patients. However, several side effects were reported that increased the risk of heart failure. We used C2C12 myoblasts to investigate the role of PPARs and their transcriptional activity during myotube formation. The role of mechanical stretch during myogenesis was also explored by applying cyclic stretch to the differentiating C2C12 myoblasts with 10% strain deformation at 1 Hz. The myogenesis medium (MM), composed of Dulbecco's modified Eagle's medium with 2% horse serum, facilitated myotube formation with increased myosin heavy chain and a-smooth muscle actin (α-SMA) protein expression. The PPARγ protein and PPAR response element (PPRE) promoter activity decreased during MM induction. Cyclic stretch further facilitated the myogenesis in MM with increased α-SMA and decreased PPARγ protein expression and inhibited PPRE promoter activity. Adding a PPARγ agonist (TZD) to the MM stopped the myogenesis and restored the PPRE promoter activity, whereas a PPARγ antagonist (GW9662) significantly increased the myotube number and length. During the myogenesis induction, application of cyclic stretch rescued the inhibitory effects of TZD. These results provide novel perspectives for mechanical stretch to interplay and rescue the dysfunction of myogenesis with the involvement of PPARγ and its target drugs.

原文English
文章編號27
期刊Frontiers in Bioengineering and Biotechnology
4
發行號MAR
DOIs
出版狀態Published - 2016 三月 21

指紋

Peroxisome Proliferator-Activated Receptors
Muscle Development
Myoblasts
Skeletal Muscle Fibers
Response Elements
Proteins
2,4-thiazolidinedione
Eagles
Myosin Heavy Chains
Muscle Proteins
Medical problems
Horses
Smooth Muscle
Glucose
Muscle
Blood Glucose
Actins
Blood
Heart Failure

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

引用此文

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title = "Cyclic stretch facilitates myogenesis in C2C12 myoblasts and rescues Thiazolidinedione-Inhibited Myotube Formation",
abstract = "Thiazolidinedione (TZD), a specific peroxisome proliferator-activated receptor γ (PPARγ) agonist, was developed to control blood glucose in diabetes patients. However, several side effects were reported that increased the risk of heart failure. We used C2C12 myoblasts to investigate the role of PPARs and their transcriptional activity during myotube formation. The role of mechanical stretch during myogenesis was also explored by applying cyclic stretch to the differentiating C2C12 myoblasts with 10{\%} strain deformation at 1 Hz. The myogenesis medium (MM), composed of Dulbecco's modified Eagle's medium with 2{\%} horse serum, facilitated myotube formation with increased myosin heavy chain and a-smooth muscle actin (α-SMA) protein expression. The PPARγ protein and PPAR response element (PPRE) promoter activity decreased during MM induction. Cyclic stretch further facilitated the myogenesis in MM with increased α-SMA and decreased PPARγ protein expression and inhibited PPRE promoter activity. Adding a PPARγ agonist (TZD) to the MM stopped the myogenesis and restored the PPRE promoter activity, whereas a PPARγ antagonist (GW9662) significantly increased the myotube number and length. During the myogenesis induction, application of cyclic stretch rescued the inhibitory effects of TZD. These results provide novel perspectives for mechanical stretch to interplay and rescue the dysfunction of myogenesis with the involvement of PPARγ and its target drugs.",
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T1 - Cyclic stretch facilitates myogenesis in C2C12 myoblasts and rescues Thiazolidinedione-Inhibited Myotube Formation

AU - Chang, Ya Ju

AU - Chen, Yun Ju

AU - Huang, Chia Wei

AU - Fan, Shih Chen

AU - Huang, Bu Miin

AU - Chang, Wen Tsan

AU - Tsai, Yau Sheng

AU - Su, Fong Chin

AU - Wu, Chia Ching

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AB - Thiazolidinedione (TZD), a specific peroxisome proliferator-activated receptor γ (PPARγ) agonist, was developed to control blood glucose in diabetes patients. However, several side effects were reported that increased the risk of heart failure. We used C2C12 myoblasts to investigate the role of PPARs and their transcriptional activity during myotube formation. The role of mechanical stretch during myogenesis was also explored by applying cyclic stretch to the differentiating C2C12 myoblasts with 10% strain deformation at 1 Hz. The myogenesis medium (MM), composed of Dulbecco's modified Eagle's medium with 2% horse serum, facilitated myotube formation with increased myosin heavy chain and a-smooth muscle actin (α-SMA) protein expression. The PPARγ protein and PPAR response element (PPRE) promoter activity decreased during MM induction. Cyclic stretch further facilitated the myogenesis in MM with increased α-SMA and decreased PPARγ protein expression and inhibited PPRE promoter activity. Adding a PPARγ agonist (TZD) to the MM stopped the myogenesis and restored the PPRE promoter activity, whereas a PPARγ antagonist (GW9662) significantly increased the myotube number and length. During the myogenesis induction, application of cyclic stretch rescued the inhibitory effects of TZD. These results provide novel perspectives for mechanical stretch to interplay and rescue the dysfunction of myogenesis with the involvement of PPARγ and its target drugs.

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