TY - JOUR
T1 - Cytokine signature of dengue patients at different severity of the disease
AU - Puc, Irwin
AU - Ho, Tzu Chuan
AU - Yen, Ko Lun
AU - Vats, Amrita
AU - Tsai, Jih Jin
AU - Chen, Po Lin
AU - Chien, Yu Wen
AU - Lo, Yu Chih
AU - Perng, Guey Chuen
N1 - Funding Information:
Funding: This research was supported, in part, by the Ministry of Education, Taiwan, R.O.C., The Aim for the Top University Project to the National Cheng Kung University (GCP), Ministry of Health and Welfare (MOHW104-CDC-C-114-114901) (GCP) and NHRI-110A1-MRCO-03212101 (JJT), and National Science Council Grant (NSC 102-2320-B-006–049, MOST 103-2320-B-006-030-MY3, MOST-106-2321-B-006 -012 -, MOST 107-2314-B-006 -063 -MY3) (GCP).
Publisher Copyright:
© 2021 by the author. Licensee MDPI, Basel, Switzerland.
PY - 2021/3/2
Y1 - 2021/3/2
N2 - Clinical presentations of dengue fever (DF) are diverse and non-specific, causing unpre-dictable progression and outcomes. Its progression and severity have been associated with cytokine levels alteration. In this study, dengue patients were classified into groups following the 2009 WHO dengue classification scheme to investigate the cytokine signature at different severity of the dis-ease: dengue without warning sign symptoms (A); dengue with warning signs (B); severe dengue (C); other fever (OF) and healthy (Healthy). We analyzed 23 different cytokines simultaneously, namely IL-1b, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-33, CD14, CD54, CD62E, CD62L, CD62p, CD106, CD121b, CD154, CD178, GM-CSF, IFN-g, MIF, ST2 and TNF from patients admitted to National Cheng Kung University Hospital during the 2015 Taiwan dengue outbreak. Cytokines TNF, CD54, CD62E, CD62L, CD62P, GM-CSF, IL-1b, IL-2, IL-6, IL-8, IL-10, IL-12p70, IL-17A, INF-g and MIF were elevated while CD106, CD154, IL-4 and L-33 were decreased when compared to the control. IL-10 demonstrated to be a potential diagnostic marker for DF (H and A group; AUC = 0.944, H and OF group; AUC = 0.969). CD121b demonstrated to be predictive of the SD (A and B group; AUC = 0.744, B and C group; AUC = 0.775). Our results demonstrate the cytokine profile changes during the progression of dengue and highlight possible biomarkers for optimizing effective intervention strategies.
AB - Clinical presentations of dengue fever (DF) are diverse and non-specific, causing unpre-dictable progression and outcomes. Its progression and severity have been associated with cytokine levels alteration. In this study, dengue patients were classified into groups following the 2009 WHO dengue classification scheme to investigate the cytokine signature at different severity of the dis-ease: dengue without warning sign symptoms (A); dengue with warning signs (B); severe dengue (C); other fever (OF) and healthy (Healthy). We analyzed 23 different cytokines simultaneously, namely IL-1b, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-33, CD14, CD54, CD62E, CD62L, CD62p, CD106, CD121b, CD154, CD178, GM-CSF, IFN-g, MIF, ST2 and TNF from patients admitted to National Cheng Kung University Hospital during the 2015 Taiwan dengue outbreak. Cytokines TNF, CD54, CD62E, CD62L, CD62P, GM-CSF, IL-1b, IL-2, IL-6, IL-8, IL-10, IL-12p70, IL-17A, INF-g and MIF were elevated while CD106, CD154, IL-4 and L-33 were decreased when compared to the control. IL-10 demonstrated to be a potential diagnostic marker for DF (H and A group; AUC = 0.944, H and OF group; AUC = 0.969). CD121b demonstrated to be predictive of the SD (A and B group; AUC = 0.744, B and C group; AUC = 0.775). Our results demonstrate the cytokine profile changes during the progression of dengue and highlight possible biomarkers for optimizing effective intervention strategies.
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U2 - 10.3390/ijms22062879
DO - 10.3390/ijms22062879
M3 - Article
C2 - 33809042
AN - SCOPUS:85102389766
SN - 1661-6596
VL - 22
SP - 1
EP - 15
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 6
M1 - 2879
ER -