Decolorization kinetics of a recombinant Escherichia coli strain harboring azo-dye-decolorizing determinants from Rhodococcus sp.

Jo-Shu Chang, Chia Yu Lin

研究成果: Article

68 引文 (Scopus)

摘要

A 6.3 kb DNA fragment containing genes responsible for azo-dye decolorization was cloned and expressed in Escherichia coli. The resulting recombinant strain E. coli CY1 decolorized 200 mg azo dye (C.I. Reactive Red 22) 1 -1 at 28 °C at 8.2 mg g cell -1 h -1 while the host (E. coli DH5α) had no color-removal activity. Addition of 0.5 mM isopropyl-β-D-thiogalacto-pyranoside (IPTG) increased the decolorization rate 3.4-fold. The dependence of the decolorization rate on initial dye concentration essentially followed Monod-type kinetics and the maximal rate occurred with the dye at 600 mg 1 -1. The decolorization rate of E. coli CY1 was optimal at 40°C and pH 11. Aeration (increased dissolved O 2 level) strongly inhibited the decolorization, but decolorization occurred effectively under static incubation conditions (no agitation was employed). The CY1 strain also exhibited excellent stability during repeated-batch operations.

原文English
頁(從 - 到)631-636
頁數6
期刊Biotechnology Letters
23
發行號8
DOIs
出版狀態Published - 2001 五月 10

指紋

Azo Compounds
Rhodococcus
Azo dyes
Escherichia coli
Kinetics
Coloring Agents
Dyes
DNA
Color
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

引用此文

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abstract = "A 6.3 kb DNA fragment containing genes responsible for azo-dye decolorization was cloned and expressed in Escherichia coli. The resulting recombinant strain E. coli CY1 decolorized 200 mg azo dye (C.I. Reactive Red 22) 1 -1 at 28 °C at 8.2 mg g cell -1 h -1 while the host (E. coli DH5α) had no color-removal activity. Addition of 0.5 mM isopropyl-β-D-thiogalacto-pyranoside (IPTG) increased the decolorization rate 3.4-fold. The dependence of the decolorization rate on initial dye concentration essentially followed Monod-type kinetics and the maximal rate occurred with the dye at 600 mg 1 -1. The decolorization rate of E. coli CY1 was optimal at 40°C and pH 11. Aeration (increased dissolved O 2 level) strongly inhibited the decolorization, but decolorization occurred effectively under static incubation conditions (no agitation was employed). The CY1 strain also exhibited excellent stability during repeated-batch operations.",
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