TY - JOUR
T1 - Dengue virus induces thrombomodulin expression in human endothelial cells and monocytes in vitro
AU - Chen, Lien Cheng
AU - Shyu, Huey Wen
AU - Lin, Hui Min
AU - Lei, Huan Yao
AU - Lin, Yee Shin
AU - Liu, Hsiao Sheng
AU - Yeh, Trai Ming
N1 - Funding Information:
This work was supported by the grant NSC96-2628-B006-006-MY3 from the National Science Council, Taiwan.
PY - 2009/5
Y1 - 2009/5
N2 - Objectives: Dengue virus (DV) infections can cause severe life-threatening dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). However, the mechanism to cause hemorrhage in DV infections remains poorly understood. Thrombomodulin (TM), expressed on the surface of endothelial cells and monocytes, is very important in regulation of coagulation and inflammation. Therefore, the effect of DV on the TM expression was studied in vitro using both endothelial cells and monocytes. Methods and results: The expression of TM in human endothelial cell line, HMEC-1, monocytic cell line THP-1 and peripheral blood mononuclear cells derived from human blood was increased after DV infection, UV-inactivated DV or recombinant DV envelop protein domain III stimulation as demonstrated by flow cytometry and immunofluorescent staining. Western blot analysis further confirmed only DV but not enterovirus 71 infection of HMEC-1 cells increased TM protein expression. In addition, RT-PCR analysis showed the increase of TM mRNA as well as other protein C activation-related molecules in DV stimulated HMEC-1 in a dose-dependent manner. Conclusion: These results suggest that DV stimulation of human endothelial cells and monocytes can increase the expression of TM, which may contribute to the anticoagulant properties of cells during DV infection.
AB - Objectives: Dengue virus (DV) infections can cause severe life-threatening dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). However, the mechanism to cause hemorrhage in DV infections remains poorly understood. Thrombomodulin (TM), expressed on the surface of endothelial cells and monocytes, is very important in regulation of coagulation and inflammation. Therefore, the effect of DV on the TM expression was studied in vitro using both endothelial cells and monocytes. Methods and results: The expression of TM in human endothelial cell line, HMEC-1, monocytic cell line THP-1 and peripheral blood mononuclear cells derived from human blood was increased after DV infection, UV-inactivated DV or recombinant DV envelop protein domain III stimulation as demonstrated by flow cytometry and immunofluorescent staining. Western blot analysis further confirmed only DV but not enterovirus 71 infection of HMEC-1 cells increased TM protein expression. In addition, RT-PCR analysis showed the increase of TM mRNA as well as other protein C activation-related molecules in DV stimulated HMEC-1 in a dose-dependent manner. Conclusion: These results suggest that DV stimulation of human endothelial cells and monocytes can increase the expression of TM, which may contribute to the anticoagulant properties of cells during DV infection.
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U2 - 10.1016/j.jinf.2009.02.018
DO - 10.1016/j.jinf.2009.02.018
M3 - Article
C2 - 19307023
AN - SCOPUS:67349185430
SN - 0163-4453
VL - 58
SP - 368
EP - 374
JO - Journal of Infection
JF - Journal of Infection
IS - 5
ER -
