Depth positioning for fast moving cells in microflow cytometry utilizing chromatic aberration under a diascopic illuminaiton scheme

Shin Yu Su, Che Hsin Lin, Lung Ming Fu

研究成果: Conference contribution

摘要

This research develops a rapid z-position measurement based on the light scattered from the passing particles under a field of light with chromatic aberration. Two band pass filters and two avalanche photodiode (APD) are used for rapid detecting the scattered light from the passing objected. The intensity ratio of two selected band 430 nm-470 nm (blue light) and 630 nm-670 nm (red light) from the scattered light becomes a significant indexes for the depth information of the detecting objects. The throughput of the developed system is capable to for detecting fast moving objects of upto 1000 particles/s which is about 10 folds compared to the spectrometer system. Human erythrocytes is also successfully detected without fluorescence labeling at a high flow velocity of 2.8 mm/s. With this approach, quantitative measurement for the depth position of rapid moving objects inside a sealed microfluidic channel can be achieved in a simple and low cost way.

原文English
主出版物標題MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
發行者Chemical and Biological Microsystems Society
頁面1825-1827
頁數3
ISBN(電子)9780979806483
出版狀態Published - 2015 一月 1
事件19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
持續時間: 2015 十月 252015 十月 29

出版系列

名字MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
國家/地區Korea, Republic of
城市Gyeongju
期間15-10-2515-10-29

All Science Journal Classification (ASJC) codes

  • 控制與系統工程

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