Depth-specific distribution and significance of nitrite-dependent anaerobic methane oxidation process in tidal flow constructed wetlands used for treating river water

Manping Zhang, Jung Chen Huang, Shanshan Sun, Muhammad Muneeb Ur Rehman, Shengbing He

研究成果: Article同行評審

27 引文 斯高帕斯(Scopus)

摘要

Tidal flow constructed wetlands (TF CWs) have been considered an effective approach to treat contaminated river water, as well as a significant role in global matter cycles, especially for carbon and nitrogen. Notably, it has been thought that methane oxidation was completely catalyzed by the aerobic process, ignoring the anaerobic methane oxidation, such as the nitrite-dependent anaerobic methane oxidation (n-damo) process. In our current work, therefore, we used molecular and stable isotopes to investigate the biodiversity, quantity and potential rate of n-damo bacteria in the TF CWs located in the Xisha Wetland Park in the Yangtze River estuary, China. The results revealed that n-damo process was active in the collected soil cores, with a decreasing trend along water depths and rates ranging from 8.48 to 23.45 nmol CO2 g 1 dry soil d 1. The n-damo bacterial contributions to CH4 oxidation and N2 production in TF CWs reached 9.49–26.26% and 20.73–47.11%, respectively, suggesting that n-damo bacteria was an important nitrogen and methane sink in the TF CWs, but had been previously overlooked. The copy numbers of total bacterial 16S rRNA and pmoA genes were 1.84–11.21 × 109 and 0.59–2.72 × 106 copies g 1 ds, respectively, as the higher abundance was found in the soil at lower water levels during tidal submergence. Diverse n-damo bacterial 16S rRNA gene sequences belonged to group B, C and D were measured, and it was found that group B and C were the most frequently measured n-damo clusters in the TF CWs. In addition, nitrite was the key factor regulating the n-damo bacterial distribution in the TF CWs. This study would broaden our horizons and help us better understand the nitrogen and methane cycles in tidal ecosystems.

原文English
文章編號137054
期刊Science of the Total Environment
716
DOIs
出版狀態Published - 2020 5月 10

All Science Journal Classification (ASJC) codes

  • 環境工程
  • 環境化學
  • 廢物管理和處置
  • 污染

指紋

深入研究「Depth-specific distribution and significance of nitrite-dependent anaerobic methane oxidation process in tidal flow constructed wetlands used for treating river water」主題。共同形成了獨特的指紋。

引用此