TY - JOUR
T1 - Design of high-resolution analysis technique for capillary electrophoresis microchip
AU - Leong, Jik Chang
AU - Tsai, Chien Hsiung
AU - Fu, Lung Ming
PY - 2007/10/9
Y1 - 2007/10/9
N2 - In this study, we develop an electrophoresis microfluidic device featuring a conventional cross-form injection system and an expansion chamber located at the inlet of the separation channel. The combined injection system/expansion chamber arrangement is designed to deliver a high-quality sample band into the separation channel such that the detection performance of the device is enhanced. The current microfluidic device is investigated using a sample of Rhodamine B dye. Also, the effects of the expansion chamber on capillary electrophoresis (CE) separation are studied by considering the separation of Hae III-digested φx-174 DNA samples. The results indicate that an expansion chamber with an expansion ratio of 2.5 and an expansion length of 500 μm delivers a sample plug with the correct shape and orientation. The microfluidic device developed in this study has an exciting potential for use in high-performance, high-throughput chemical analysis applications and in many other applications throughout the field of micro-total-analysis systems.
AB - In this study, we develop an electrophoresis microfluidic device featuring a conventional cross-form injection system and an expansion chamber located at the inlet of the separation channel. The combined injection system/expansion chamber arrangement is designed to deliver a high-quality sample band into the separation channel such that the detection performance of the device is enhanced. The current microfluidic device is investigated using a sample of Rhodamine B dye. Also, the effects of the expansion chamber on capillary electrophoresis (CE) separation are studied by considering the separation of Hae III-digested φx-174 DNA samples. The results indicate that an expansion chamber with an expansion ratio of 2.5 and an expansion length of 500 μm delivers a sample plug with the correct shape and orientation. The microfluidic device developed in this study has an exciting potential for use in high-performance, high-throughput chemical analysis applications and in many other applications throughout the field of micro-total-analysis systems.
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U2 - 10.1143/JJAP.46.6865
DO - 10.1143/JJAP.46.6865
M3 - Article
AN - SCOPUS:35348856214
VL - 46
SP - 6865
EP - 6870
JO - Japanese Journal of Applied Physics
JF - Japanese Journal of Applied Physics
SN - 0021-4922
IS - 10 A
ER -