Detect the different isoforms using GeneChip human exon 1.0 ST arrays

Mi Chia Ma, Hsiao Fang Lin, Ying Xu

研究成果: Conference contribution

摘要

Alternative splicing is the RNA splicing variation mechanism. The exon isoforms lead to numerous different expressions through the alternative splicing. It's imperative to find the carcinogenic gene from the massive quantity of genes and understand the expressed isoforms. In this paper, the statistical procedures are applied on the colon cancer sample data of Affymetrix GeneChip Human Exon 1.0 ST Array and the dataset is used as the example of finding isoforms. First, the Wilcoxon sign rank test was applied to choose the suspect carcinogenic genes. About 54,854 suspect genes are selected from original 312,368 genes at 5% significant level. Second, one sample t-test and the factor analysis are used to estimate the tumor and normal isoforms for each suspect carcinogenic gene, respectively. Furthermore, the confirmations are made by comparing the estimated results with Alternative Splicing Database. Finally, the results may be provided to the biologist to make biological confirmations and discover the new isoforms.

原文English
主出版物標題Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
DOIs
出版狀態Published - 2009
事件2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009 - Tianjin, China
持續時間: 2009 十月 172009 十月 19

出版系列

名字Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009

Other

Other2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
國家/地區China
城市Tianjin
期間09-10-1709-10-19

All Science Journal Classification (ASJC) codes

  • 生物醫學工程
  • 健康資訊學
  • 健康資訊管理

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