Detection and distribution of endogenous steroids in human stratum corneum

Shu Ping Tseng, Cheng-Chan Lu, Pao-Chi Liao, Chen-Hsi Chou, Hamm Ming Sheu, Jui-Chen Tsai

研究成果: Article

2 引文 (Scopus)

摘要

Objectives The objective of the study was to investigate the presence and distribution of endogenous steroids in human stratum corneum (SC) with respect to sex, age, anatomical site, and depth into SC, using a noninvasive sampling technique and a sensitive analytic method for quantitation. Materials and methods Corneocytes in the SC samples removed by sequential tape stripping from the forearm, forehead, and back sites were processed and analyzed using a validated liquid chromatography-tandem mass spectrometry method for the quantitation of hydrocortisone, cortisone, and testosterone. Results In the 32 volunteers surveyed, testosterone was only detected at the forearm site in a single volunteer. Both hydrocortisone and cortisone were detected in 5-7 individuals out of 16 from both the age 20-35 years and age 50-65 years groups. Maximal amounts of hydrocortisone and cortisone found in SC of forehead, forearm, and back were 0.37 ng/cm2, 0.96 ng/cm2, and 0.49 ng/cm2; and 0.20 ng/cm2, 0.12 ng/cm2, and 0.06 ng/cm2, respectively, and were either higher than or comparable to those reported in human hair in terms of concentration by SC weight. In the population with either hydrocortisone or cortisone detected, no significant differences relating to sex, age groups, and anatomical sites were observed for the amount of hydrocortisone and cortisone in the SC. However, significantly higher amount of cortisone was found in the surface layers of SC than deeper layers in the age 50-65 years group. Conclusion The results demonstrate that, with the achievable sensitivity of current analytical technology, physiological concentrations of endogenous steroids, such as hydrocortisone and cortisone, can be found in the SC of some individuals.

原文English
頁(從 - 到)19-24
頁數6
期刊Dermatologica Sinica
32
發行號1
DOIs
出版狀態Published - 2014 三月 1

指紋

Cortisone
Cornea
Steroids
Hydrocortisone
Forearm
Forehead
Testosterone
Volunteers
Tandem Mass Spectrometry
Liquid Chromatography
Hair
Age Groups
Technology
Weights and Measures
Population

All Science Journal Classification (ASJC) codes

  • Dermatology

引用此文

Tseng, Shu Ping ; Lu, Cheng-Chan ; Liao, Pao-Chi ; Chou, Chen-Hsi ; Sheu, Hamm Ming ; Tsai, Jui-Chen. / Detection and distribution of endogenous steroids in human stratum corneum. 於: Dermatologica Sinica. 2014 ; 卷 32, 編號 1. 頁 19-24.
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abstract = "Objectives The objective of the study was to investigate the presence and distribution of endogenous steroids in human stratum corneum (SC) with respect to sex, age, anatomical site, and depth into SC, using a noninvasive sampling technique and a sensitive analytic method for quantitation. Materials and methods Corneocytes in the SC samples removed by sequential tape stripping from the forearm, forehead, and back sites were processed and analyzed using a validated liquid chromatography-tandem mass spectrometry method for the quantitation of hydrocortisone, cortisone, and testosterone. Results In the 32 volunteers surveyed, testosterone was only detected at the forearm site in a single volunteer. Both hydrocortisone and cortisone were detected in 5-7 individuals out of 16 from both the age 20-35 years and age 50-65 years groups. Maximal amounts of hydrocortisone and cortisone found in SC of forehead, forearm, and back were 0.37 ng/cm2, 0.96 ng/cm2, and 0.49 ng/cm2; and 0.20 ng/cm2, 0.12 ng/cm2, and 0.06 ng/cm2, respectively, and were either higher than or comparable to those reported in human hair in terms of concentration by SC weight. In the population with either hydrocortisone or cortisone detected, no significant differences relating to sex, age groups, and anatomical sites were observed for the amount of hydrocortisone and cortisone in the SC. However, significantly higher amount of cortisone was found in the surface layers of SC than deeper layers in the age 50-65 years group. Conclusion The results demonstrate that, with the achievable sensitivity of current analytical technology, physiological concentrations of endogenous steroids, such as hydrocortisone and cortisone, can be found in the SC of some individuals.",
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Detection and distribution of endogenous steroids in human stratum corneum. / Tseng, Shu Ping; Lu, Cheng-Chan; Liao, Pao-Chi; Chou, Chen-Hsi; Sheu, Hamm Ming; Tsai, Jui-Chen.

於: Dermatologica Sinica, 卷 32, 編號 1, 01.03.2014, p. 19-24.

研究成果: Article

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T1 - Detection and distribution of endogenous steroids in human stratum corneum

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AU - Lu, Cheng-Chan

AU - Liao, Pao-Chi

AU - Chou, Chen-Hsi

AU - Sheu, Hamm Ming

AU - Tsai, Jui-Chen

PY - 2014/3/1

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N2 - Objectives The objective of the study was to investigate the presence and distribution of endogenous steroids in human stratum corneum (SC) with respect to sex, age, anatomical site, and depth into SC, using a noninvasive sampling technique and a sensitive analytic method for quantitation. Materials and methods Corneocytes in the SC samples removed by sequential tape stripping from the forearm, forehead, and back sites were processed and analyzed using a validated liquid chromatography-tandem mass spectrometry method for the quantitation of hydrocortisone, cortisone, and testosterone. Results In the 32 volunteers surveyed, testosterone was only detected at the forearm site in a single volunteer. Both hydrocortisone and cortisone were detected in 5-7 individuals out of 16 from both the age 20-35 years and age 50-65 years groups. Maximal amounts of hydrocortisone and cortisone found in SC of forehead, forearm, and back were 0.37 ng/cm2, 0.96 ng/cm2, and 0.49 ng/cm2; and 0.20 ng/cm2, 0.12 ng/cm2, and 0.06 ng/cm2, respectively, and were either higher than or comparable to those reported in human hair in terms of concentration by SC weight. In the population with either hydrocortisone or cortisone detected, no significant differences relating to sex, age groups, and anatomical sites were observed for the amount of hydrocortisone and cortisone in the SC. However, significantly higher amount of cortisone was found in the surface layers of SC than deeper layers in the age 50-65 years group. Conclusion The results demonstrate that, with the achievable sensitivity of current analytical technology, physiological concentrations of endogenous steroids, such as hydrocortisone and cortisone, can be found in the SC of some individuals.

AB - Objectives The objective of the study was to investigate the presence and distribution of endogenous steroids in human stratum corneum (SC) with respect to sex, age, anatomical site, and depth into SC, using a noninvasive sampling technique and a sensitive analytic method for quantitation. Materials and methods Corneocytes in the SC samples removed by sequential tape stripping from the forearm, forehead, and back sites were processed and analyzed using a validated liquid chromatography-tandem mass spectrometry method for the quantitation of hydrocortisone, cortisone, and testosterone. Results In the 32 volunteers surveyed, testosterone was only detected at the forearm site in a single volunteer. Both hydrocortisone and cortisone were detected in 5-7 individuals out of 16 from both the age 20-35 years and age 50-65 years groups. Maximal amounts of hydrocortisone and cortisone found in SC of forehead, forearm, and back were 0.37 ng/cm2, 0.96 ng/cm2, and 0.49 ng/cm2; and 0.20 ng/cm2, 0.12 ng/cm2, and 0.06 ng/cm2, respectively, and were either higher than or comparable to those reported in human hair in terms of concentration by SC weight. In the population with either hydrocortisone or cortisone detected, no significant differences relating to sex, age groups, and anatomical sites were observed for the amount of hydrocortisone and cortisone in the SC. However, significantly higher amount of cortisone was found in the surface layers of SC than deeper layers in the age 50-65 years group. Conclusion The results demonstrate that, with the achievable sensitivity of current analytical technology, physiological concentrations of endogenous steroids, such as hydrocortisone and cortisone, can be found in the SC of some individuals.

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