Detection of lipopolysaccharide binding peptides by the use of a lipopolysaccharide-coated piezoelectric crystal biosensor

研究成果: Article同行評審

25 引文 斯高帕斯(Scopus)

摘要

A method has been developed to monitor the interaction between a lipopolysaccharide (LPS) and LPS-binding peptides using a piezoelectric quartz crystal (PQC). Different pH conditions were evaluated to coat LPS onto AT-cut crystals that had been sputtered with gold and carboxylated with a 4,4-dithiodi(n-butyric acid). The optimal pH for LPS coating onto the crystal was 4-5. Synthetic peptides that represent different regions of human bactericidal/permeability-increasing protein, BPI (BPI 85-99, BPI90-101, BPI 157-167) and polymyxin B (PmB) as well as negative control peptide (HBsAg 139-147) were utilized to compare their binding ability to this LPS-coated PQC sensor. The results showed that PmB gave the greatest decrease to the resonant frequency indicating greatest binding ability. BPI 85-99, considered the main part of the LPS binding domain of BPI, was the next greatest, while BPI 157-167 and HBsAg 139-147 showed little response. In addition, BPI 90-101 and PmB mimicking peptide showed intermediate LPS-binding ability, which was less than that of BPI 85-99, but was higher than that of BPI 157-167. These results suggest the PQC biosensor is potentially useful for the detection and comparison of the LPS-binding ability of different peptides by using an LPS-coated piezoelectric crystal.

原文English
頁(從 - 到)49-54
頁數6
期刊Analytica Chimica Acta
340
發行號1-3
DOIs
出版狀態Published - 1997 三月 20

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物化學
  • 環境化學
  • 光譜

指紋

深入研究「Detection of lipopolysaccharide binding peptides by the use of a lipopolysaccharide-coated piezoelectric crystal biosensor」主題。共同形成了獨特的指紋。

引用此