Determination of optimum gene transfection conditions using the Taguchi method for an electroporation microchip

Sheng Chung Yang, Keng Shiang Huang, Hung Yi Chen, Yu Cheng Lin

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)

摘要

This study demonstrates the optimization of an electroporation (EP) microchip system for gene transfection using the Taguchi method. The computer-aided engineering simulations show that approximately 80% of the cells are distributed in the middle region of the reaction chamber and are subjected to the applied electric field. A smaller electrode gap, such as 50 μm, gained a larger and more uniform electric field distribution in the middle region. The parameters of the EP microchip system, which can have an influence on the transfecting rate were optimized, resulting in values of 50 μm electrode gap, 20 μg/mL pEGFP-N1 concentration, 6 V applied voltage, and 2-pulse. A larger-the-better Ω transformation was employed for better additivity. The analyzed results of interactions A × C, A × D, and C × D show that there were strong interactions on level 3 of factors A, C, and D among each other. Using the optimized EP microchip system, the great majority of pEGFP-N1 plasmids was successfully transfected into the NIH Swiss mouse embryo fibroblast cell lines (NIH-3T3). The optimized EP parameters helped with improving the EP microchip system and elevating the average transfection rate from 17.2 to 40.36%, and the inaccuracy of the predicted transfection rate of 43.67% was as small as 3.31%.

原文English
頁(從 - 到)551-557
頁數7
期刊Sensors and Actuators, B: Chemical
132
發行號2
DOIs
出版狀態Published - 2008 六月 16

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學

指紋

深入研究「Determination of optimum gene transfection conditions using the Taguchi method for an electroporation microchip」主題。共同形成了獨特的指紋。

引用此