Determination of tadalafil in small volumes of plasma by high-performance liquid chromatography with UV detection

Ching Ling Cheng, Chen Hsi Chou

研究成果: Article同行評審

47 引文 斯高帕斯(Scopus)

摘要

Tadalafil is a potent reversible phosphodiesterase-5 inhibitor used for the treatment of erectile dysfunction. This study describes a simple and sensitive high-performance liquid chromatographic (HPLC) method for the determination of tadalafil in 50 μl of rat plasma. Tadalafil and the internal standard lamotrigine were extracted with 0.5 ml of tert-butyl methyl ether, after the samples alkalinized with 20 μl of sodium hydroxide solution (1N). Chromatographic separation was achieved on a C18 column with the mobile phase of acetonitrile-water containing 20 mM phosphate buffer (pH 7) (35/65, v/v), at a flow rate of 1 ml/min. The eluant was detected at 290 nm. The retention time was about 4.5 min for lamotrigine and 15 min for tadalafil. No endogenous substances were found to interfere. Calibration curves were linear from 10 to 2000 ng/ml. The recovery of tadalafil from plasma was greater than 77%. The limit of quantitation was 10 ng/ml. The intra- and inter-day imprecision (expressed as coefficient of variation, C.V.) did not exceed 10.7%, and the accuracy was within 5.9% deviation of the nominal concentration. The method is suitable in pharmacokinetic investigation and monitoring tadalafil concentration.

原文English
頁(從 - 到)278-284
頁數7
期刊Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
822
發行號1-2
DOIs
出版狀態Published - 2005 8月 5

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物化學
  • 臨床生物化學
  • 細胞生物學

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