The development of e-book creates a revolutionary of knowledge dissemination and changes the way of teaching and learning. More and more studies have established that learning by e-book is an effective approach to enhance reading comprehension. On the other hand, learning is an actively processes of cognition, including attention, comprehension, perception, and organization. Past researches had been established that learner's reading attention and anxiety influenced learner's performance obviously. In tradition, it was very difficult for a teacher to maintain every student's learning status effectively. However, in recent years, the relationship between brain and education had been heeded because there was a strong correlation between them. But holding a brainwave experiment in the past was required a lot of preparations and equipment settings. Hence, this study aimed to develop a neurofeedback-based e-book system to maintain an effective learning status through improving reading attention and reducing reading anxiety. It also provided a web-based interface to help teacher to observe student's reading concentration and detect his/her learning status. Finally, an expert interview was adopted to ensure the effectiveness of neurofeedback proposed in this study. Five experts were enrolled in this interview. They stated several important suggestions of neurofeedback, e-book, teaching, and learning phase to improve this e-book system in the future.