In the present study, a complete training curriculum was proposed according to the content of occupational health program mandated by the law and job demands of the safety and health personnel, and was finalized by the focus group. The developed training courses include 41 courses in total and could be classified into the four categories of the hazard recognition, hazard evaluation, hazard controls, and environmental management, and three levels of the introductory, advanced and professional level. The needs of proposed courses were assessed from the organization, and person analysis aspects. The training needs obtained from the organization analysis aspect were served as guidelines for adjusting the prioritization of training needs for course of different levels in different categories obtained from the person analysis aspect. As a result, top 11training courses were identified and were recommended for conducting on-the-job training for the safety and hygiene workforce in nanotechnology industries to reduce the incidence of occupational illness.