Development of a hierarchical oligonucleotide primer extension assay for the qualitative and quantitative analysis of Cylindrospermopsis raciborskii subspecies in freshwater

Jer Horng Wu, Mao Hsuang Hsu, Ching Hui Hung, I. Cheng Tseng, Tsair Fuh Lin

研究成果: Article同行評審

7 引文 斯高帕斯(Scopus)

摘要

A newly-developed molecular method, hierarchical oligonucleotide primer extension (HOPE), was used to analyze various groups within the species Cylindrospermopsis raciborskii. PCR-amplified internally transcribed spacer sequences of 16S-23S from C. raciborskii in reservoir samples of Taiwan and Kinmen were examined. One of eight sequevars in the clone libraries was closely related to strains obtained from the European continent, while the others, designated of Taiwan (TW) type, belonged to a novel group. Optimized HOPE analyses showed that C. raciborskii distributed in different reservoirs with a relative abundance of 0.5% to 76.4% in the cyanobacterial communities. They further detected the concurrence of three C. raciborskii subpopulations, in which European and TW groups were predominant. The TW sequevars accounted for greater than 87.5% of C. raciborskii in the reservoirs Taihu, Yangmin, Jinsha, and Mudan, while this decreased to 55.4-58.1%, accompanied by a proportional increase of the European group, in reservoirs Lantan and Renyi. These findings revealed the complex subspecies structure within C. raciborskii and the subspecies dynamics associated with geographic locations.

原文English
頁(從 - 到)103-110
頁數8
期刊Microbes and Environments
25
發行號2
DOIs
出版狀態Published - 2010

All Science Journal Classification (ASJC) codes

  • 生態學、進化論、行為學與系統學
  • 土壤科學
  • 植物科學

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