Development of a MR-Visible Compound for Tracing Neuroanatomical Connections In Vivo

Carolyn W.H. Wu, Olga Vasalatiy, Ning Liu, Haitao Wu, Sarah Cheal, Der Yow Chen, Alan P. Koretsky, Gary L. Griffiths, Roger B.H. Tootell, Leslie G. Ungerleider

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16 引文 斯高帕斯(Scopus)


Traditional studies of neuroanatomical connections require injection of tracer compounds into living brains, then histology of the postmortem tissue. Here, we describe and validate a compound that reveals neuronal connections in vivo, using MRI. The classic anatomical tracer CTB (cholera-toxin subunit-B) was conjugated with a gadolinium-chelate to form GdDOTA-CTB. GdDOTA-CTB was injected into the primary somatosensory cortex (S1) or the olfactory pathway of rats. High-resolution MR images were collected at a range of time points at 11.7T and 7T. The transported GdDOTA-CTB was visible for at least 1 month post-injection, clearing within 2 months. Control injections of non-conjugated GdDOTA into S1 were not transported and cleared within 1-2 days. Control injections of Gd-Albumin were not transported either, clearing within 7 days. These MR results were verified by classic immunohistochemical staining for CTB, in the same animals. The GdDOTA-CTB neuronal transport was target specific, monosynaptic, stable for several weeks, and reproducible.

頁(從 - 到)229-243
出版狀態Published - 2011 四月 28

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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