Development of an image-based algorithm for the motility characterizations of the nematode caenorhabditis elegans

Wan Jung Kuo, Han Sheng Chuang

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

The nematode Caenorhabditis (C.) elegans has been widely used as a model animal for fundamental biological research. In order to investigate the effect of exercise on degenerative behaviors of C. elegans, such as physiological (lifespan, progeny) and biomechanical (propulsion, total power and swimming gait) properties, we developed a flow visualization technique to characterize the motility of C. elegans. Quantifying the motility of micro-organisms is always essential in understanding their biomechanical properties. Up to date, however, the direct measurement of the motility of C. elegans remains a big challenge due to lack of proper tools. Therefore, a simple image-based algorithm using a microparticle image velocimetry (μPIV) for deriving the kinetic power and propulsive force of the nematode C. elegans was developed in this study. For the measurement, each worm was confined in a 0.5 μL droplet which was sandwiched between two glass slides separated by two tapes. The motility of the confined worm was derived from the fluid motion according to the law of conservation of energy. The experimental result showed that our measured values appear to be in good agreements with the prior data. The image-based algorithm was proven to be a simple and automated measure for characterizing the dynamic motility of micro-swimmers. The study will eventually provide valuable information for treating and preventing degenerative diseases in higher animals.

原文English
主出版物標題1st Global Conference on Biomedical Engineering and 9th Asian-Pacific Conference on Medical and Biological Engineering
編輯Fong-Chin Su, Ming-Long Yeh, Shyh-Hau Wang
發行者Springer Science and Business Media Deutschland GmbH
頁面107-110
頁數4
ISBN(電子)9783319122618
DOIs
出版狀態Published - 2015
事件1st Global Conference on Biomedical Engineering, GCBME 2014 and 9th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2014 - Tainan, Taiwan
持續時間: 2014 10月 92014 10月 12

出版系列

名字IFMBE Proceedings
47
ISSN(列印)1680-0737
ISSN(電子)1433-9277

Other

Other1st Global Conference on Biomedical Engineering, GCBME 2014 and 9th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2014
國家/地區Taiwan
城市Tainan
期間14-10-0914-10-12

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 生物醫學工程

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