Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila

Jih Guang Yao, Bonnie M. Weasner, Lan Hsin Wang, Chuen-Chuen Jang, Brandon Weasner, Chiou Yang Tang, Claire L. Salzer, Chun Hong Chen, Bruce Hay, Y. Henry Sun, Justin P. Kumar

研究成果: Article

27 引文 (Scopus)

摘要

In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.

原文English
頁(從 - 到)535-551
頁數17
期刊Developmental Biology
315
發行號2
DOIs
出版狀態Published - 2008 三月 15

指紋

Toes
Diptera
Drosophila
Drosophila melanogaster
Genes
Vertebrates
Retina
Dissection
Fruit
Protein Isoforms
Proteins
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

引用此文

Yao, Jih Guang ; Weasner, Bonnie M. ; Wang, Lan Hsin ; Jang, Chuen-Chuen ; Weasner, Brandon ; Tang, Chiou Yang ; Salzer, Claire L. ; Chen, Chun Hong ; Hay, Bruce ; Sun, Y. Henry ; Kumar, Justin P. / Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila. 於: Developmental Biology. 2008 ; 卷 315, 編號 2. 頁 535-551.
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Yao, JG, Weasner, BM, Wang, LH, Jang, C-C, Weasner, B, Tang, CY, Salzer, CL, Chen, CH, Hay, B, Sun, YH & Kumar, JP 2008, 'Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila', Developmental Biology, 卷 315, 編號 2, 頁 535-551. https://doi.org/10.1016/j.ydbio.2007.12.037

Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila. / Yao, Jih Guang; Weasner, Bonnie M.; Wang, Lan Hsin; Jang, Chuen-Chuen; Weasner, Brandon; Tang, Chiou Yang; Salzer, Claire L.; Chen, Chun Hong; Hay, Bruce; Sun, Y. Henry; Kumar, Justin P.

於: Developmental Biology, 卷 315, 編號 2, 15.03.2008, p. 535-551.

研究成果: Article

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AU - Wang, Lan Hsin

AU - Jang, Chuen-Chuen

AU - Weasner, Brandon

AU - Tang, Chiou Yang

AU - Salzer, Claire L.

AU - Chen, Chun Hong

AU - Hay, Bruce

AU - Sun, Y. Henry

AU - Kumar, Justin P.

PY - 2008/3/15

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N2 - In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.

AB - In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.

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