Differentially expressed genes correlated with fibrosis in a rat model of chronic partial bladder outlet obstruction

Yuan-Shuo Hsueh, Hui Hua Chang, Shun Yao Ko, Yi Pai Lin, Wei Yu Lin

研究成果: Article同行評審

摘要

Chronic partial bladder outlet obstruction (PBOO) is a prevalent clinical problem that may result from multiple etiologies. PBOO may be a secondary condition to various anatomical and functional abnormalities. Bladder fibrosis is the worst outcome of PBOO. However, gene alterations and the mechanism of fibrosis development after PBOO onset are not clear. Therefore, we aimed to investigate gene expression alterations during chronic PBOO. A rat model of PBOO was established and validated by a significant increase in rat bladder weight. The bladder samples were further analyzed by microarray, and differentially expressed genes (DEGs) that are more related to PBOO compared with the control genes were selected. The data showed that 16 significantly upregulated mRNAs and 3 significantly downregulated mRNAs are involved in fibrosis. Moreover, 13 significantly upregulated mRNAs and 12 significantly downregulated mRNAs are related to TGFB signaling. Twenty-two significantly upregulated mRNAs and nine significantly downregulated mRNAs are related to the extracellular matrix. The genes with differential expressions greater than four-fold included Grem1, Thbs1, Col8a1, Itga5, Tnc, Lox, Timp1, Col4a1, Col4a2, Bhlhe40, Itga1, Tgfb3, and Gadd45b. The gene with a differential expression less than a quarter-fold was Thbs2. These findings show the potential roles of these genes in the physiology of PBOO.

原文English
文章編號2219
期刊Processes
9
發行號12
DOIs
出版狀態Published - 2021 12月

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 化學工程(雜項)
  • 製程化學與技術

指紋

深入研究「Differentially expressed genes correlated with fibrosis in a rat model of chronic partial bladder outlet obstruction」主題。共同形成了獨特的指紋。

引用此