Direct binding of Concanvalin A onto iron oxide nanoparticles for fast magnetic selective separation of lactoferrin

Bo Hung Lai, Chih Hao Chang, Chun Chieh Yeh, Dong Hwang Chen

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)

摘要

Concanavalin A (Con A) was successfully bound onto Fe3O 4 magnetic nanoparticles (MNPs) via carbodiimide activation as a magnetic nano-adsorbent for glycoprotein separation. The resulting Con A-bound Fe3O4 MNPs were nearly superparamagnetic with a mean diameter of 11.74 ± 3.86 nm. The size and structure of Fe 3O4 MNPs had no significant changes after Con A binding. The binding of Con A on the surface of Fe3O4 MNPs was confirmed by the analysis of Fourier transform infrared spectroscopy. From the thermogravimetric analysis and the concentration change of Con A in the binding solution, the weight percentage of Con A in the typical Con A-bound Fe 3O4 MNPs was determined to be about 10 wt%. Furthermore, the Con A-bound Fe3O4 MNPs were found to be quite efficient for the fast adsorption of lactoferrin. The equilibrium was achieved within 5 min and the adsorption data for lactoferrin obeyed the Langmuir isotherm equation. At pH 7 and 25 °C, the maximum adsorption capacity and equilibrium constant were 59.2 mg/g and 0.0103 L/mg, respectively. In addition, by SDS-polyacrylamide gel electrophoresis, the capability of Con A-bound Fe 3O4 MNPs for the selective adsorption of lactoferrin was further confirmed.

原文English
頁(從 - 到)83-88
頁數6
期刊Separation and Purification Technology
108
DOIs
出版狀態Published - 2013

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 篩選和分離

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