Disassociation of σ subunit from RNA polymerase of Xanthomonas oryzae pv. oryzae by phage Xp10 infection

Shan Hua Lin, Jun Sheng Liu, Bei Chang Yang, Tsong Teh Kuo

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

The σ subunit of Xanthomonas oryzae pv. oryzae is disassociated from host RNA polymerase after phage Xp10 infection. To clarify the possible mechanism for this observation, σ subunit was purified and an antiserum against σ subunit was prepared. Immunoprecipitation of RNA polymerase by the anti-core RNA polymerase antiserum, followed by immunoblotting with anti-σ subunit antibody, revealed that σ subunit was lost from RNA polymerase within 10 minutes after Xp10 infection. Loss of σ subunit was not observed under other stress conditions including heat and cold stress, starvation and growth to stationary phase. Two-dimensional immunoblotting analysis did not reveal any covalent modification of either σ subunit or RNA polymerase after Xp10 infection. These results suggest that separation of σ subunit from RNA polymerase may be due to competition with other binding factors.

原文English
頁(從 - 到)9-15
頁數7
期刊FEMS Microbiology Letters
162
發行號1
DOIs
出版狀態Published - 1998 5月 1

All Science Journal Classification (ASJC) codes

  • 微生物學
  • 分子生物學
  • 遺傳學

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