Distinct regulation of gene expression by prostaglandin F (PGF) is associated with PGF resistance or susceptibility in human granulosa-luteal cells

Shaw Jenq Tsai, Meng Hsing Wu, Pei Chin Chuang, Hsiu Mei Chen

研究成果: Article同行評審

23 引文 斯高帕斯(Scopus)

摘要

The effects of human chorionic gonadotrophin (HCG) and prostaglandin F (PGF) on regulation of human granulosa-luteal cell (GLC) function at different stages of differentiation (day 2 versus day 8 of culture) were studied. Expression of LH receptor mRNA and biosynthesis of progesterone were HCG dependent in human GLC at all stages (n=6,P < 0.05). Steady-state concentrations of mRNA encoding for FP (a specific high-affinity plasma membrane receptor for PGF) were not dependent on, but were stimulated by, addition of HCG (10 IU/ ml) or 8-bromo-cAMP (0.5 mmol/l) (n=6, P < 0.05). Treatment with PGF (100 nmol/l) decreased FP mRNA concentration, but had no effect on LH receptor and cyclo oxygenase-2 (COX-2) expression on day 2 of cultured GLC (n = 8). As a result, the progesterone biosynthesis by GLC was not affected. On day 8, PGF induced FP and PGHS-2 expression and at the same time decreased LH receptor expression, resulting in inhibition of progesterone output by GLC. Our data demonstrated that early stage GLC (day 2 of culture) are resistant to PGF-induced inhibition of progesterone synthesis but underwent further differentiation and acquired luteolytic capacity after 8 days culture in vitro. We conclude that, via distinct gene regulation at different stages of differentiation, human GLC may become resistant or susceptible to PGF-induced luteolysis.

原文English
頁(從 - 到)415-423
頁數9
期刊Molecular Human Reproduction
7
發行號5
DOIs
出版狀態Published - 2001 五月

All Science Journal Classification (ASJC) codes

  • 生殖醫學
  • 胚胎學
  • 分子生物學
  • 遺傳學
  • 婦產科
  • 發展生物學
  • 細胞生物學

指紋

深入研究「Distinct regulation of gene expression by prostaglandin F<sub>2α</sub> (PGF<sub>2α</sub>) is associated with PGF<sub>2α</sub> resistance or susceptibility in human granulosa-luteal cells」主題。共同形成了獨特的指紋。

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