Docosahexaenoic acid inhibits synaptic transmission and epileptiform activity in the rat hippocampus

Chainllie Young, Po Wu Gean, Lih Chu Chiou, Yu Zen Shen

研究成果: Article

73 引文 斯高帕斯(Scopus)

摘要

Docosahexaenoic acid (DHA) has been suggested to be required for neuronal development and synaptic plasticity. However, in view of the fact that DHA facilitates NMDA responses and blocks K+ channels, it might predispose the neurons to epileptiform bursting. By using extracellular recording of population spikes in the CA1 region of rat hippocampal slices, we tested this possibility by examining the effect of DHA on the epileptiform activity induced by bicuculline or in Mg2+-free medium. When stimuli were delivered to the Schaffer collateral/commissural pathway every 20 or 30 sec, DHA had no significant effect on the epileptiform activity. However, when the frequency of stimulation was increased to 0.2 Hz, DHA attenuated the amplitude of the bursting activity induced by bicuculline to 57.5 ± 10.8% and those induced by Mg2+-free ACSF to 65.8 ± 13.9% of control. DHA reduced the slope of field excitatory postsynaptic potential (fEPSP) to 77.1 ± 7.4% of baseline, without significant effect on the ratio of paired-pulse facilitation (PPF). By intracellular recording of neurons in the stratum pyramidale of rat hippocampal slices, we found that DHA markedly inhibited the repetitive firing of action potentials elicited by depolarizing current pulses but did not affect the initial action potential. Thus, DHA may attenuate epileptic activity mainly through the frequency-dependent blockade of Na+ channels. (C) 2000 Wiley-Liss, Inc.

原文English
頁(從 - 到)90-94
頁數5
期刊Synapse
37
發行號2
DOIs
出版狀態Published - 2000 七月 22

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

指紋 深入研究「Docosahexaenoic acid inhibits synaptic transmission and epileptiform activity in the rat hippocampus」主題。共同形成了獨特的指紋。

  • 引用此