Docosahexaenoic acid production from crude glycerol by Schizochytrium limacinum SR21

In our search for a substitute energy source, microalgal biodiesel has presented itself as a potential candidate. However, the development of biodiesel results in the overproduction of crude glycerol, which can cause undesirable environmental issues. The environmental harm can be minimized by converting crude glycerol into value-added products. One solution involves using a microalga Schizochytrium limacinum SR21 to convert crude glycerol into docosahexaenoic acid (DHA). DHA is an essential fatty acid, necessary for developing brain functions in infants and maintaining healthy brain activity in adults. In our study, the highest DHA productivity of 233.73 mg/g biomass was obtained using 3 % crude glycerol in Medium 2 at 20 °C under mixo/heterotrophic cultivation.