Downregulation of SIRT1 and GADD45G genes and left atrial fibrosis induced by right ventricular-dependent pacing in a complete atrioventricular block pig model

Wei Chieh Lee, Yu Sheng Lin, Man Jing Chen, Wan Chun Ho, Huang Chung Chen, Tzu Hao Chang, Ping Yen Liu, Mien Cheng Chen

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)

摘要

The molecular and genetic mechanisms underlying left atrial (LA) enlargement and atrial fibrosis following right ventricular (RV)-dependent pacing remain unclear. Our objective was to investigate genetic expressions in the LA of pigs subjected to RV pacing for atrioventricular block (AVB), as well as to identify the differential gene expressions affected by biventricular (BiV) pacing. We established an AVB pig model and divided the subjects into three groups: a sham control group, an RV pacing group, and a BiV pacing group. Differential expression genes (DEGs) analyses conducted through next-generation sequencing (NGS) and enrichment analyses were employed to identify genes with altered expression in the LA myocardium. The RV pacing group showed a significant increase in extracellular fibrosis in the LA myocardium compared to the control group. NGS analysis revealed suppressed expression of the sirtuin signaling pathway in the RV pacing group. Among the DEGs within this pathway, GADD45G was found to be downregulated in the RV pacing group and upregulated in the BiV pacing group. Remarkably, the BiV pacing group exhibited elevated levels of GADD45G protein. In our study, we observed significant downregulation of SIRT1 and GADD45G genes, which are associated with the sirtuin signaling pathway, in the LA myocardium of the RV pacing group when compared to the control group. Moreover, these genes, which were downregulated in the RV pacing group, displayed a noteworthy upregulation in the BiV pacing group when compared to the RV pacing group.

原文English
頁(從 - 到)360-373
頁數14
期刊Biomolecules and Biomedicine
24
發行號2
DOIs
出版狀態Published - 2024 3月 11

All Science Journal Classification (ASJC) codes

  • 一般生物化學,遺傳學和分子生物學
  • 一般醫學

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