Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System

Chih Hung Wang; Keng Fu Hsu; Gwo Bin Lee

Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System

Chih Hung Wang
, Keng Fu Hsu
, Gwo Bin Lee

婦產學科

研究成果: Conference contribution

摘要

A new screening strategy has been developed to select a new aptamer suitably used in a clinical environment by using an in vivo-like systematic evolution of ligands by exponential enrichment (SELEX) following by conventional position and negative selection processes. One novel methylation-specific aptamer with high affinity and high specificity was successfully selected such that it could capture methylated tumor circulating cell-free DNA (cfDNA) from plasma of ovarian cancer (OvCa) patients. The aptamer was conjugated onto magnetic beads and applied in an integrated microfluidic chip for detection and quantification of two methylated tumor suppressor genes, BRCA1/BRCA2, from cfDNA in plasma or blood.

原文	English
主出版物標題	2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
發行者	Institute of Electrical and Electronics Engineers Inc.
頁面	160-163
頁數	4
ISBN（電子）	9784886864352
出版狀態	Published - 2023
事件	22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023 - Kyoto, Japan 持續時間: 2023 6月 25 → 2023 6月 29

出版系列

名字	2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

Conference

Conference	22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
國家/地區	Japan
城市	Kyoto
期間	23-06-25 → 23-06-29

UN SDG

此研究成果有助於以下永續發展目標

SDG 3 良好的健康和福祉

All Science Journal Classification (ASJC) codes

電腦科學應用
電氣與電子工程
控制和優化
儀器

其它文件與鏈接

引用此

Wang, C. H., Hsu, K. F., & Lee, G. B. (2023). Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. 於 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023 (頁 160-163). (2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023). Institute of Electrical and Electronics Engineers Inc..

Wang, Chih Hung ; Hsu, Keng Fu ; Lee, Gwo Bin. / Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Institute of Electrical and Electronics Engineers Inc., 2023. 頁 160-163 (2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023).

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title = "Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System",

abstract = "A new screening strategy has been developed to select a new aptamer suitably used in a clinical environment by using an in vivo-like systematic evolution of ligands by exponential enrichment (SELEX) following by conventional position and negative selection processes. One novel methylation-specific aptamer with high affinity and high specificity was successfully selected such that it could capture methylated tumor circulating cell-free DNA (cfDNA) from plasma of ovarian cancer (OvCa) patients. The aptamer was conjugated onto magnetic beads and applied in an integrated microfluidic chip for detection and quantification of two methylated tumor suppressor genes, BRCA1/BRCA2, from cfDNA in plasma or blood.",

author = "Wang, \{Chih Hung\} and Hsu, \{Keng Fu\} and Lee, \{Gwo Bin\}",

note = "Publisher Copyright: {\textcopyright} 2023 IEEJ.; 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023 ; Conference date: 25-06-2023 Through 29-06-2023",

year = "2023",

language = "English",

series = "2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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address = "United States",

}

Wang, CH, Hsu, KF & Lee, GB 2023, Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. 於 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023, Institute of Electrical and Electronics Engineers Inc., 頁 160-163, 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023, Kyoto, Japan, 23-06-25.

Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. / Wang, Chih Hung; Hsu, Keng Fu; Lee, Gwo Bin.
2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Institute of Electrical and Electronics Engineers Inc., 2023. p. 160-163 (2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023).

研究成果: Conference contribution

TY - GEN

T1 - Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System

AU - Wang, Chih Hung

AU - Hsu, Keng Fu

AU - Lee, Gwo Bin

PY - 2023

Y1 - 2023

N2 - A new screening strategy has been developed to select a new aptamer suitably used in a clinical environment by using an in vivo-like systematic evolution of ligands by exponential enrichment (SELEX) following by conventional position and negative selection processes. One novel methylation-specific aptamer with high affinity and high specificity was successfully selected such that it could capture methylated tumor circulating cell-free DNA (cfDNA) from plasma of ovarian cancer (OvCa) patients. The aptamer was conjugated onto magnetic beads and applied in an integrated microfluidic chip for detection and quantification of two methylated tumor suppressor genes, BRCA1/BRCA2, from cfDNA in plasma or blood.

AB - A new screening strategy has been developed to select a new aptamer suitably used in a clinical environment by using an in vivo-like systematic evolution of ligands by exponential enrichment (SELEX) following by conventional position and negative selection processes. One novel methylation-specific aptamer with high affinity and high specificity was successfully selected such that it could capture methylated tumor circulating cell-free DNA (cfDNA) from plasma of ovarian cancer (OvCa) patients. The aptamer was conjugated onto magnetic beads and applied in an integrated microfluidic chip for detection and quantification of two methylated tumor suppressor genes, BRCA1/BRCA2, from cfDNA in plasma or blood.

UR - https://www.scopus.com/pages/publications/85193517519

UR - https://www.scopus.com/pages/publications/85193517519#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85193517519

T3 - 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

SP - 160

EP - 163

BT - 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

Y2 - 25 June 2023 through 29 June 2023

ER -

Wang CH, Hsu KF, Lee GB. Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. 於 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Institute of Electrical and Electronics Engineers Inc. 2023. p. 160-163. (2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023).

Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System

摘要

出版系列

Conference

UN SDG

All Science Journal Classification (ASJC) codes

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指紋

引用此