Duplicated C-Class MADS-Box genes reveal distinct roles in gynostemium development in cymbidium ensifolium (Orchidaceae)

Shih Yu Wang, Pei Fang Lee, Yung I. Lee, Yu Yun Hsiao, You Yi Chen, Zhao Jun Pan, Zhong Jian Liu, Wen Chieh Tsai

研究成果: Article同行評審

44 引文 斯高帕斯(Scopus)

摘要

The orchid floral organs represent novel and effective structures for attracting pollination vectors. In addition, to avoid inbreeding, the androecium and gynoecium are united in a single structure termed the gynostemium. Identification of C-class MADS-box genes regulating reproductive organ development could help determine the level of homology with the current ABC model of floral organ identity in orchids. In this study, we isolated and characterized two C-class AGAMOUS-like genes, denoted CeMADS1 and CeMADS2, from Cymbidium ensifolium. These two genes showed distinct spatial and temporal expression profiles, which suggests their functional diversification during gynostemium development. Furthermore, the expression of CeMADS1 but not CeMADS2 was eliminated in the multitepal mutant whose gynostemium is replaced by a newly emerged flower, and this ecotopic flower continues to produce sepals and petals centripetally. Protein interaction relationships among CeMADS1, CeMADS2 and E-class PeMADS8 proteins were assessed by yeast two-hybrid analysis. Both CeMADS1 and CeMADS2 formed homodimers and heterodimers with each other and the E-class PeMADS protein. Furthermore, transgenic Arabidopsis plants overexpressing CeMADS1 or CeMADS2 showed limited growth of primary inflorescence. Thus, CeMADS1 may have a pivotal C function in reproductive organ development in C. ensifolium.

原文English
頁(從 - 到)563-577
頁數15
期刊Plant and Cell Physiology
52
發行號3
DOIs
出版狀態Published - 2011 三月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 植物科學
  • 細胞生物學

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