EcVig, a novel grouper immune-gene associated with antiviral activity against NNV infection

Ying Chun Yeh, Yi Jiou Hsu, Yi Min Chen, Han You Lin, Huey Lang Yang, Tzong Yueh Chen, Han Ching Wang

研究成果: Article

9 引文 (Scopus)

摘要

VHSV-induced genes (VIGs) were first identified in rainbow trout ( Oncorhynchus mykiss) and subsequently isolated in a variety of fish. Recent studies have shown that most VIGs have immunological functions against pathogenic infections. However, most research has focused on Vig1, such that our present understanding of these genes in other fish species remains limited. This study isolated a homologue of the uncharacterized O. mykiss Vig-B319 (EcVig) from orange-spotted grouper ( Epinephelus coioides). Genomic organization suggests that four EcVig isoforms (EcVig A-D), are generated through alternative splicing. Due to the encoding of 2 immunoglobulin (Ig) domains, the EcVig protein can be considered a member of the immunoglobulin superfamily. The expression of EcVig increased 3. days after hatching (dph) and peaked at 9. dph. This pattern is similar to that displayed by EcMx, an important grouper antiviral gene. Additionally, a tissue tropism assay revealed that EcVig A is the major EcVig isoform present in the tissues considered by this study, with the expression of EcVig A exceeding that of EcVig B. We subsequently investigated whether EcVig expression was induced by the viral pathogen nervous necrosis virus (NNV) or the bacterial pathogen Vibrio anguillarum. Following injection with NNV, the expression levels of EcVig showed significant up-regulation. Conversely, a significant reduction was observed in EcVig expression in brain samples collected from V. anguillarum injected grouper. The overexpression of EcVig A suppressed the replication of NNV in grouper GF-1 cell lines, suggesting that EcVig is an important antiviral factor in the grouper immune responses.

原文English
頁(從 - 到)68-75
頁數8
期刊Developmental and Comparative Immunology
43
發行號1
DOIs
出版狀態Published - 2014 三月 1

指紋

Virus Diseases
Antiviral Agents
Oncorhynchus mykiss
Necrosis
Viruses
Genes
Protein Isoforms
Fishes
Tropism
Vibrio
Alternative Splicing
Immunoglobulins
Up-Regulation
Cell Line
Injections
Brain
Infection
Research
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

引用此文

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abstract = "VHSV-induced genes (VIGs) were first identified in rainbow trout ( Oncorhynchus mykiss) and subsequently isolated in a variety of fish. Recent studies have shown that most VIGs have immunological functions against pathogenic infections. However, most research has focused on Vig1, such that our present understanding of these genes in other fish species remains limited. This study isolated a homologue of the uncharacterized O. mykiss Vig-B319 (EcVig) from orange-spotted grouper ( Epinephelus coioides). Genomic organization suggests that four EcVig isoforms (EcVig A-D), are generated through alternative splicing. Due to the encoding of 2 immunoglobulin (Ig) domains, the EcVig protein can be considered a member of the immunoglobulin superfamily. The expression of EcVig increased 3. days after hatching (dph) and peaked at 9. dph. This pattern is similar to that displayed by EcMx, an important grouper antiviral gene. Additionally, a tissue tropism assay revealed that EcVig A is the major EcVig isoform present in the tissues considered by this study, with the expression of EcVig A exceeding that of EcVig B. We subsequently investigated whether EcVig expression was induced by the viral pathogen nervous necrosis virus (NNV) or the bacterial pathogen Vibrio anguillarum. Following injection with NNV, the expression levels of EcVig showed significant up-regulation. Conversely, a significant reduction was observed in EcVig expression in brain samples collected from V. anguillarum injected grouper. The overexpression of EcVig A suppressed the replication of NNV in grouper GF-1 cell lines, suggesting that EcVig is an important antiviral factor in the grouper immune responses.",
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EcVig, a novel grouper immune-gene associated with antiviral activity against NNV infection. / Yeh, Ying Chun; Hsu, Yi Jiou; Chen, Yi Min; Lin, Han You; Yang, Huey Lang; Chen, Tzong Yueh; Wang, Han Ching.

於: Developmental and Comparative Immunology, 卷 43, 編號 1, 01.03.2014, p. 68-75.

研究成果: Article

TY - JOUR

T1 - EcVig, a novel grouper immune-gene associated with antiviral activity against NNV infection

AU - Yeh, Ying Chun

AU - Hsu, Yi Jiou

AU - Chen, Yi Min

AU - Lin, Han You

AU - Yang, Huey Lang

AU - Chen, Tzong Yueh

AU - Wang, Han Ching

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AB - VHSV-induced genes (VIGs) were first identified in rainbow trout ( Oncorhynchus mykiss) and subsequently isolated in a variety of fish. Recent studies have shown that most VIGs have immunological functions against pathogenic infections. However, most research has focused on Vig1, such that our present understanding of these genes in other fish species remains limited. This study isolated a homologue of the uncharacterized O. mykiss Vig-B319 (EcVig) from orange-spotted grouper ( Epinephelus coioides). Genomic organization suggests that four EcVig isoforms (EcVig A-D), are generated through alternative splicing. Due to the encoding of 2 immunoglobulin (Ig) domains, the EcVig protein can be considered a member of the immunoglobulin superfamily. The expression of EcVig increased 3. days after hatching (dph) and peaked at 9. dph. This pattern is similar to that displayed by EcMx, an important grouper antiviral gene. Additionally, a tissue tropism assay revealed that EcVig A is the major EcVig isoform present in the tissues considered by this study, with the expression of EcVig A exceeding that of EcVig B. We subsequently investigated whether EcVig expression was induced by the viral pathogen nervous necrosis virus (NNV) or the bacterial pathogen Vibrio anguillarum. Following injection with NNV, the expression levels of EcVig showed significant up-regulation. Conversely, a significant reduction was observed in EcVig expression in brain samples collected from V. anguillarum injected grouper. The overexpression of EcVig A suppressed the replication of NNV in grouper GF-1 cell lines, suggesting that EcVig is an important antiviral factor in the grouper immune responses.

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