EcVig, a novel grouper immune-gene associated with antiviral activity against NNV infection

Ying Chun Yeh, Yi Jiou Hsu, Yi Min Chen, Han You Lin, Huey Lang Yang, Tzong Yueh Chen, Han Ching Wang

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)


VHSV-induced genes (VIGs) were first identified in rainbow trout ( Oncorhynchus mykiss) and subsequently isolated in a variety of fish. Recent studies have shown that most VIGs have immunological functions against pathogenic infections. However, most research has focused on Vig1, such that our present understanding of these genes in other fish species remains limited. This study isolated a homologue of the uncharacterized O. mykiss Vig-B319 (EcVig) from orange-spotted grouper ( Epinephelus coioides). Genomic organization suggests that four EcVig isoforms (EcVig A-D), are generated through alternative splicing. Due to the encoding of 2 immunoglobulin (Ig) domains, the EcVig protein can be considered a member of the immunoglobulin superfamily. The expression of EcVig increased 3. days after hatching (dph) and peaked at 9. dph. This pattern is similar to that displayed by EcMx, an important grouper antiviral gene. Additionally, a tissue tropism assay revealed that EcVig A is the major EcVig isoform present in the tissues considered by this study, with the expression of EcVig A exceeding that of EcVig B. We subsequently investigated whether EcVig expression was induced by the viral pathogen nervous necrosis virus (NNV) or the bacterial pathogen Vibrio anguillarum. Following injection with NNV, the expression levels of EcVig showed significant up-regulation. Conversely, a significant reduction was observed in EcVig expression in brain samples collected from V. anguillarum injected grouper. The overexpression of EcVig A suppressed the replication of NNV in grouper GF-1 cell lines, suggesting that EcVig is an important antiviral factor in the grouper immune responses.

頁(從 - 到)68-75
期刊Developmental and Comparative Immunology
出版狀態Published - 2014 3月

All Science Journal Classification (ASJC) codes

  • 免疫學
  • 發展生物學


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