TY - JOUR
T1 - Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat
AU - Rowe, Justin
AU - Chen, Qingshan
AU - Domire, Zachary J.
AU - McCullough, Matthew B.
AU - Sieck, Gary
AU - Zhan, Wen Zhi
AU - An, Kai Nan
N1 - Funding Information:
This study is supported by National Institute of Health (NIH) grant EB00812 . The study sponsors had no role in study design, collection, analysis and interpretation of data, wiring of the manuscript, or decision to submit the manuscript for publication.
PY - 2010/1
Y1 - 2010/1
N2 - Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle's passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.
AB - Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle's passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.
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U2 - 10.1016/j.medengphy.2009.11.002
DO - 10.1016/j.medengphy.2009.11.002
M3 - Article
C2 - 19945332
AN - SCOPUS:72449166430
SN - 1350-4533
VL - 32
SP - 90
EP - 94
JO - Medical Engineering and Physics
JF - Medical Engineering and Physics
IS - 1
ER -