In recent years, atmospheric micro-plasma has various applications in the biomedical field, including blood coagulation, tissue sterilization, wound care etc. However, the mechanisms of atmospheric micro-plasma, mainly through particle excited state, and some of the gas molecules enter into the target is still controversy. In many experiments, micro-plasma exposure into cells medium, are reflected in the amount of excess plasma species, so that the cells will accelerate the process of apoptosis, and even necrosis. Therefore diagnosis plasma parameters is necessary, in this study the distance from the plasma jet to the target, different kind of gas addition, or various kind of solution will be applied. The purpose of this research is to explore the interaction of plasma parameter to the solution compositions for further optimal applications in wound care or cancer treatment.