Effect of ultrasound doses on the amyloid-β 25-35 induced PC12 apoptosis

Khun Yi Chiu, Show Huie Chen, Shyh Hau Wang

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

Previous studies have shown that Amyloid-β(Aβ) may induce the apoptosis of neuronal cells leading to the syndrome of Alzheimer's disease (AD). The stimulation by optical energy was found able to greatly inhibit Aβinduced apoptosis. This study aims to further explore the effect of different doses of ultrasonic insonification on neuronal cells. Experiments were carried out using PC12 cells added with Aβ 25-35 of a 20 μM during pre-cultured preparation. These cells were respectively stimulated by a single and multiple insonification for three minutes with a 20% duty cycle ultrasound of the intensity of 150 mW/cm2 (SATA).- The cellular response was assessed, using the microscopic morphology, cell death measured by the typical MTT assay, and annexin V/PI double stain assay, for 8 times within 72 hours after that cells were stimulated. Results showed that both stimulations by single and multiple does ultrasound may diminish Aβ induced neuronal cells apoptosis. The diminish effects tend to be time dependent corresponding to 72 and 12 hours after ultrasound exposure by single and multiple insonification, respectively. Fluorescence stain results indicated that those cells stimulated by a single dose ultrasound tended to slightly inhibit Aβ-induced PC12 to apoptosis. This study demonstrated that the effect of diminishing neuronal cells from apoptosis could be regulated with the insonation of appropriate ultrasonic doses.

原文English
主出版物標題29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
頁面5837-5840
頁數4
DOIs
出版狀態Published - 2007 十二月 1
事件29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07 - Lyon, France
持續時間: 2007 八月 232007 八月 26

出版系列

名字Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN(列印)0589-1019

Other

Other29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
國家France
城市Lyon
期間07-08-2307-08-26

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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