Effect of various ultrasound insonifications on the Aβ-induced apoptotic neurons

Chun Yi Chiu, Shyh-Hau Wang, Show Huie Chen

研究成果: Conference contribution

摘要

Amyloid beta (Aβ) is a peptide that was known able to induce the apoptosis of nerve cells in the brain leading to the development of Alzheimer's disease (AD). In addition to providing medication treatment for the neurodegenerative disease, previous cell culture studies applying physical stimulations of a certain optical energy or low intensity ultrasound on the apoptotic cells have demonstrated that the viability of Ab-induced apoptotic neurons could be increased. Current study is to further explore insonification effects, such as frequency, exposure duration, and intensity on the apoptotic nerve cells and that the result is to provide a basic understanding about the therapeutic effects of ultrasound on the Ab-induced apoptotic neurons. Experiment were carried out using neuronal PC12 cells that were insonified by 1 MHz ultrasound with 20% duty cycle, 150 mW/cm2 (SATA) for 3 minutes. After 72 hours, the viability and fate of cells were respectively evaluated by MTT assay and annexin V/PI double stain assay. Results showed that the stimulations by low intensity ultrasounds may contribute to the reduction of A-b induced cell apoptosis or necrosis. The effect of decreasing apoptotic cells tends to be apparent at a certain time depending on the condition of insonified ultrasounds. The variation of temperature in the cell plate was within 1°C throughout each insonification procedure. Thus, the effect on apoptotic PC12 cells may be largely due to the non- thermal effects of insonified ultrasound, which could enhance the permeability and other signal transduction pathways for cells.

原文English
主出版物標題2007 IEEE Ultrasonics Symposium Proceedings, IUS
頁面1772-1775
頁數4
DOIs
出版狀態Published - 2007 十二月 1
事件2007 IEEE Ultrasonics Symposium, IUS - New York, NY, United States
持續時間: 2007 十月 282007 十月 31

出版系列

名字Proceedings - IEEE Ultrasonics Symposium
ISSN(列印)1051-0117

Other

Other2007 IEEE Ultrasonics Symposium, IUS
國家/地區United States
城市New York, NY
期間07-10-2807-10-31

All Science Journal Classification (ASJC) codes

  • 聲學與超音波

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