Effects of granulation tissue conditioned medium on the in vitro differentiation of keratinocytes

C. C. Huang, Z. X. Yi, W. Y. Chao

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)

摘要

The accumulation of desquamated keratinizing squamous epithelial cells appears to be a crucial factor in the pathogenesis of middle ear cholesteatomas. The accumulation of keratin debris is due to the proliferation and the terminal differentiation of basal keratinocytes. Since cholesteatomas are usually associated with inflammatory reactions in the middle ear cavity, we examined the effects of a granulation tissue conditioned medium on the terminal differentiation of basal keratinocytes in vitro. This conditioned medium stimulated the terminal differentiation of basal keratinocytes by showing: (a) increased incorporation of 3H-leucine into cell envelopes; (b) an increased number of SDS-insoluble cell envelopes; and (c) increased transglutaminase activity (as a marker for terminal cellular differentiation). Our present studies further suggest that inflammatory granulation tissue plays an important role in the clinical growth and development of the cholesteatoma.

原文English
頁(從 - 到)325-329
頁數5
期刊Archives of Oto-Rhino-Laryngology
245
發行號6
DOIs
出版狀態Published - 1988 十二月

All Science Journal Classification (ASJC) codes

  • 耳鼻咽喉科

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