Effects of malonate C60 derivatives on activated microglia

Shun Fen Tzeng, Jia Ling Lee, Jon Sun Kuo, Chung Shi Yang, Periyagamy Murugan, Lin Ai Tai, Kuo Chu Hwang

研究成果: Article同行評審

22 引文 斯高帕斯(Scopus)

摘要

Activated microglia in acute and chronic neurodegenerative disease of the central nervous system (CNS) can produce large amounts of free radicals, such as reactive oxygen species (ROS), which subsequently contribute to neuropathogenesis. Thus, it is believed that the induction of microglial deactivation can reduce neuronal injury. Buckminsterfullerene (C60) derivatives that possess free radical scavenging properties have been demonstrated to prevent neuronal cell death caused by excitotoxic insult. In this study, we investigated the biological role of two malonic acid C60 derivatives referred as trans-2 and trans-3 on microglia in the presence of the endotoxin lipopolysaccharide (LPS). Treatment of LPS-activated microglia with trans-2 and trans-3 induced a significant degree of transformation of amoeboid microglia to the ramified phenotype. To understand the mechanism underlying this C60 mediated microglial morphological transformation, we examined the production of proinflammatory cytokines, interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), as well as the final NO products (nitrate and nitrite) in the microglial culture supernatant. Although inducible nitric oxide (iNOS) mRNA and protein expression in LPS-activated microglia were slightly decreased by trans-2 and trans-3, levels of nitrate and nitrite were unaffected. Paradoxically, trans-2 and trans-3 were found to increase the release of IL-1β in the activated microglial culture. However, trans-2 and trans-3 improved the activity of the antioxidant enzyme, superoxide dismutase (SOD) in LPS-treated microglia. Therefore, our results suggest that the C60 derivatives might increase microglial SOD enzymatic activity which causes microglial morphological transformation from the activated amoeboid phenotype to the resting ramified form.

原文English
頁(從 - 到)61-68
頁數8
期刊Brain Research
940
發行號1-2
DOIs
出版狀態Published - 2002 六月 14

All Science Journal Classification (ASJC) codes

  • 神經科學 (全部)
  • 分子生物學
  • 神經病學(臨床)
  • 發展生物學

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