Effects of passivation treatments on titanium alloy with nanometric scale roughness and induced changes in fibroblast initial adhesion evaluated by a cytodetacher

C. C. Wang, Y. C. Hsu, Fong-chin Su, S. C. Lu, Tzer-Min Lee

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)

摘要

Passivation treatments of titanium alloy alter not only its nanosurface characteristics of oxides and ion release but also surface roughness (Ra), and wettability as well, where nanosurface characteristics of oxides include chemistries of oxides, amphoteric-OH groups adsorbed on oxides, and oxide thickness. Consequently, the passivation treatment affects the alloy's cyto-comparability. In this study, we polish specimens to achieve nanometric scale roughness. In addition, treatment effects are evaluated for surface topology, roughness, wettability, and responses of fibroblasts consisting of MTT assay, initial adhesion strength, and morphology. The initial adhesion strength is measured using a cytodetacher that achieves nano-Newton resolution. Results reveal that (1) the treatment effects on the percentage of Ti-OH basic groups and wettability are nearly collinear; (2) the Ra of passivated Ti-6Al-4V ranges from 1.9 to 7.4 nm; (3) the initial adhesion strength of fibroblast ranges from 58 to 143 nN, and it is negatively correlated to the Ra; (4) the passivation results in distinguishable morphologies, which further substantiate the negative correlation between cell initial adhesion force and Ra; and (5) our results fall short of confirming previous reports that found positively charged functional groups promoting fibroblast attachment and spread. Potential causes of the inconsistency are addressed.

原文English
頁(從 - 到)370-383
頁數14
期刊Journal of Biomedical Materials Research - Part A
88
發行號2
DOIs
出版狀態Published - 2009 二月 1

All Science Journal Classification (ASJC) codes

  • 陶瓷和複合材料
  • 生物材料
  • 生物醫學工程
  • 金屬和合金

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