Effects of pipette modulation and imaging distances on ion currents measured with Scanning Ion Conductance Microscopy (SICM)

Chiao Chen Chen, Lane A. Baker

研究成果: Article同行評審

38 引文 斯高帕斯(Scopus)

摘要

Local conductance variations can be estimated by measuring ion current magnitudes with scanning ion conductance microscopy (SICM). Factors which influence image quality and quantitation of ion currents measured with SICM have been evaluated. Specifically, effects of probe-sample separation and pipette modulation have been systematically studied for the case of imaging conductance variations at pores in a polymer membrane under transmembrane concentration gradients. The influence of probe-sample separation on ion current images was evaluated using distance-modulated (ac) feedback. Approach curves obtained using non-modulated (dc) feedback were also recorded to determine the relative influence of pipette-generated convection by comparison of ion currents measured with both ac and dc feedback modes. To better interpret results obtained, comparison to a model based on a disk-shaped geometry for nanopores in the membrane, as well as relevant position-dependent parameters of the experiment is described. These results advance our current understanding of conductance measurements with SICM.

原文English
頁(從 - 到)90-97
頁數8
期刊Analyst
136
發行號1
DOIs
出版狀態Published - 2011 一月 7

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物化學
  • 環境化學
  • 光譜
  • 電化學

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